Controlling Protein Enrichment in Lipid Sponge Phase Droplets using SNAP-Tag Bioconjugation

Autor:	Mahta Moinpour, Alessandro Fracassi, Roberto J. Brea, Marta Salvador‐Castell, Sudip Pandey, Madison M. Edwards, Soenke Seifert, Simpson Joseph, Sunil K. Sinha, Neal K. Devaraj
Rok vydání:	2021
Předmět:	O(6)-Methylguanine-DNA Methyltransferase Organic Chemistry Molecular Medicine Proteins Lipid Droplets Molecular Biology Biochemistry Phospholipids
Zdroj:	Chembiochem : a European journal of chemical biology. 23(5)
ISSN:	1439-7633
Popis:	All cells use organized lipid compartments to facilitate specific biological functions. Membrane-bound organelles create defined spatial environments that favor unique chemical reactions while isolating incompatible biological processes. Despite the fundamental role of cellular organelles, there is a scarcity of methods for preparing functional artificial lipid-based compartments. Here, we demonstrate a robust bioconjugation system for sequestering proteins into zwitterionic lipid sponge phase droplets. Incorporation of benzylguanine (BG)-modified phospholipids that form stable covalent linkages with an O
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ca4034566ba8d9c46a933a129f0ed690 https://pubmed.ncbi.nlm.nih.gov/34936727 Zobrazit plný text záznamu Plný text