Adaptation of the white-rot basidiomycete Panus tigrinus for transformation of high concentrations of chlorophenols
| Autor: | Alexey A. Leontievsky, N. M. Myasoedova, Ludmila A. Golovleva, Christine S. Evans, Mohammad R. Sedarati |
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| Rok vydání: | 2002 |
| Předmět: |
Fungus
Applied Microbiology and Biotechnology chemistry.chemical_compound Bioreactors Bioreactor Food science Phenols Chromatography High Pressure Liquid biology Ecology Basidiomycota General Medicine Biodegradation biology.organism_classification Adaptation Physiological Culture Media Pentachlorophenol Biodegradation Environmental Peroxidases chemistry biology.protein Chlorophenols Biotechnology Peroxidase Toxicant |
| Zdroj: | Applied Microbiology and Biotechnology. 59:599-604 |
| ISSN: | 1432-0614 0175-7598 |
| Popis: | During feed-batch cultivation of the white-rot fungus Panus tigrinus in a 5-l bioreactor on N-limited medium, 100, 200, 500, 1,000 and 2,000 mg 2,4,6-trichlorophenol (2,4,6-TCP) l(-1) were added sequentially after 90% removal of the previous portion of the toxicant. The addition of 500 mg 2,4,6-TCP l(-1) without preliminary adaptation killed the culture. The addition of 300 mg 2,4,6-TCP l(-1) without prior adaptation resulted in its slower removal than removal of 2,000 mg 2,4,6-TCP l(-1) by this adapted culture. After adaptation of P. tigrinus to 2,4,6-TCP in a 72-l bioreactor, the mixture of 2,4-dichlorophenol, 2,4,6-TCP, and pentachlorophenol, each at 500 mg x l(-1), was totally removed over 3 weeks. No lignin peroxidase activity was found in the course of cultivation of the fungus. Laccase activity was suppressed by addition of 2,4,6-TCP. Mn-peroxidase was found to be responsible for transformation of the chlorophenols. As final products of the process, several newly formed aromatic polymers, both chlorinated and non-chlorinated, were found in the culture liquid. |
| Databáze: | OpenAIRE |
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