Complete genome sequence analysis of dengue virus type 2 isolated in Brunei
| Autor: | Mun Yik Fong, Osmali Osman, Shamala Devi |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Serotype
Cancer Research Brunei Base pair viruses Molecular Sequence Data Genome Viral Dengue virus Biology medicine.disease_cause Genome Virus Dengue fever Cell Line Disease Outbreaks Dengue Aedes Virology Genotype medicine Animals Humans Phylogeny DNA Primers Genetics Sequence Analysis DNA Dengue Virus medicine.disease digestive system diseases Infectious Diseases Primer (molecular biology) |
| Zdroj: | Virus research. 135(1) |
| ISSN: | 0168-1702 |
| Popis: | In a previous study, we have reported the detection and isolation of dengue virus in Brunei (Osman, O., Fong, M.Y., Devi, S., 2007. A preliminary study of dengue infection in Brunei. JJID 60 (4), 205–208). DEN-2 was the predominant serotype followed by DEN-1. The full genomic sequences of 3 DEN-2 viruses isolated during the 2005–2006 dengue incident in Brunei were determined. Twenty-five primer sets were designed to amplify contiguous overlapping fragments of approximately 500–600 base pairs spanning the entire sequence of the viral genome. The amplified PCR products were sent for sequencing and their nucleotides and the deduced amino acids were determined. All three DEN-2 virus isolated were clustered in the Cosmopolitan genotype of the DEN-2 classification by Twiddy et al. This work constitutes the first complete genetic characterization of three Brunei DEN-2 virus strains. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect