Trans-splicing and RNA editing of LSU rRNA in Diplonema mitochondria
| Autor: | Matus Valach, Sandrine Moreira, Gertraud Burger, Georgette N. Kiethega |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
RNA
Mitochondrial 5.8S ribosomal RNA Molecular Sequence Data Euglenozoa Biology MT-RNR1 Trans-Splicing 03 medical and health sciences 5S ribosomal RNA 0302 clinical medicine 23S ribosomal RNA Genetics RNA Antisense Internal transcribed spacer 030304 developmental biology 0303 health sciences Base Sequence Intron Ribosomal RNA Ribosome Subunits Large Eukaryotic Mitochondria RNA editing RNA Ribosomal Nucleic Acid Conformation RNA RNA Editing 030217 neurology & neurosurgery |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 |
| Popis: | Mitochondrial ribosomal RNAs (rRNAs) often display reduced size and deviant secondary structure, and sometimes are fragmented, as are their corresponding genes. Here we report a mitochondrial large subunit rRNA (mt-LSU rRNA) with unprecedented features. In the protist Diplonema, the rnl gene is split into two pieces (modules 1 and 2, 534- and 352-nt long) that are encoded by distinct mitochondrial chromosomes, yet the rRNA is continuous. To reconstruct the post-transcriptional maturation pathway of this rRNA, we have catalogued transcript intermediates by deep RNA sequencing and RT-PCR. Gene modules are transcribed separately. Subsequently, transcripts are end-processed, the module-1 transcript is polyuridylated and the module-2 transcript is polyadenylated. The two modules are joined via trans-splicing that retains at the junction ∼26 uridines, resulting in an extent of insertion RNA editing not observed before in any system. The A-tail of trans-spliced molecules is shorter than that of mono-module 2, and completely absent from mitoribosome-associated mt-LSU rRNA. We also characterize putative antisense transcripts. Antisense-mono-modules corroborate bi-directional transcription of chromosomes. Antisense-mt-LSU rRNA, if functional, has the potential of guiding concomitantly trans-splicing and editing of this rRNA. Together, these findings open a window on the investigation of complex regulatory networks that orchestrate multiple and biochemically diverse post-transcriptional events. |
| Databáze: | OpenAIRE |
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