Regulation of nuclear factor-κB (NF-κB) activity and apoptosis by estradiol in bovine granulosa cells
| Autor: | Kelli E. Valdez, Adele M. Turzillo |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
endocrine system
medicine.medical_specialty Programmed cell death media_common.quotation_subject Apoptosis Biology Biochemistry chemistry.chemical_compound Endocrinology Ovarian Follicle Annexin Internal medicine Follicular phase medicine Animals Molecular Biology Ovulation media_common Granulosa Cells TUNEL assay Estradiol NF-kappa B NF-κB Follicular fluid Enzyme Activation chemistry Cattle Female |
| Zdroj: | Molecular and Cellular Endocrinology. 243:66-73 |
| ISSN: | 0303-7207 |
| DOI: | 10.1016/j.mce.2005.09.004 |
| Popis: | Although atresia of bovine follicles is associated with apoptosis of granulosa cells, the signals initiating this cell death have not been resolved. NF-kappaB has been implicated as an important regulator of genes controlling apoptosis, and previous studies indicate that estradiol may modulate NF-kappaB activation. We hypothesized that estradiol activates NF-kappaB, and thus, inhibits apoptosis in granulosa cells of dominant follicles. Dominant follicles were collected on Days 4, 6, and 8 of the first follicular wave following ovulation and assayed for NF-kappaB activity. Concentrations of estradiol in follicular fluid decreased on Day 6 compared to Day 4 of the wave. NF-kappaB activity in granulosa cells was lower on Day 8 compared to Days 4 and 6. In vitro, NF-kappaB activity and minimal incidence of apoptosis ( |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect