Flow cytometric-based protocols for assessing anti-MT-2 IgG1 reactivity: High-dimensional data handling to define predictors for clinical follow-up of Human T-cell Leukemia virus type-1 infection
Autor: | Andréa Teixeira-Carvalho, Marcelo Antônio Pascoal-Xavier, Denise Utsch Gonçalves, Olindo Assis Martins-Filho, Vanessa Peruhype-Magalhães, João Gabriel Ramos Ribas, Ludmila Melo Cardoso, Anna Bárbara F. Carneiro-Proietti, Juan Jonathan-Gonçalves, Ágata Lopes Ribeiro, Laurence Rodrigues do Amaral, Jordana Grazziela Coelho-dos-Reis, Matheus de Souza Gomes, Ana Lúcia Borges Starling, Giph |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Time Factors 030106 microbiology Immunology Asymptomatic Cell Line Flow cytometry Diagnosis Differential 03 medical and health sciences Myelopathy Predictive Value of Tests immune system diseases Tropical spastic paraparesis medicine Cluster Analysis Data Mining Humans Immunology and Allergy Fluorescent Antibody Technique Indirect Human T-lymphotropic virus 1 medicine.diagnostic_test Deltaretrovirus Antibodies business.industry Decision Trees Reproducibility of Results virus diseases Viral Load Flow Cytometry medicine.disease HTLV-I Infections Paraparesis Tropical Spastic High-Throughput Screening Assays Human T cell leukemia virus Serum cytokine 030104 developmental biology Case-Control Studies Immunoglobulin G Clinical diagnosis Asymptomatic Diseases Disease Progression Cytokines Differential diagnosis medicine.symptom business Algorithms Biomarkers |
Zdroj: | Journal of Immunological Methods. 444:36-46 |
ISSN: | 0022-1759 |
Popis: | The present work provides an innovative methodological approach to assess the anti-HTLV-1 IgG1 reactivity with practical application in clinical laboratory. Serum from non-infected healthy controls (NI) and HTLV-1-infected patients, categorized as asymptomatic (AS), putatively progressing to HTLV-1 associated myelopathy/tropical spastic paraparesis - HAM/TSP (pHAM) or with clinical diagnosis of HAM/TSP (HT) were assayed in two-parallel flow cytometry platforms, referred as: Fix and FixPerm protocols. Operating-characteristics analysis indicated that a single pair of attributes ("serum dilution/cut-off") for Fix and FixPerm protocols presented excellent performance for the diagnosis of HTLV-1 infection. Conversely, Fix and FixPerm protocols displayed weak/moderate overall performances when applied with prognosis purposes of HTLV-1 infection. A panoramic snapshot provided by the reactivity boards revealed clearly the higher sensitivity of FixPerm protocol for detecting seropositivity for HT, suggesting that stepwise combinatory criteria would improve the global performance of using a single pair of attributes. Three data mining strategies were tested, including endpoint titer analysis, heatmap assemblage and decision tree analysis. Bi-dimensional heatmap analysis demonstrated that, while the clustering profile of NI vs HTLV-1 |
Databáze: | OpenAIRE |
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