Flow cytometric-based protocols for assessing anti-MT-2 IgG1 reactivity: High-dimensional data handling to define predictors for clinical follow-up of Human T-cell Leukemia virus type-1 infection

Autor: Andréa Teixeira-Carvalho, Marcelo Antônio Pascoal-Xavier, Denise Utsch Gonçalves, Olindo Assis Martins-Filho, Vanessa Peruhype-Magalhães, João Gabriel Ramos Ribas, Ludmila Melo Cardoso, Anna Bárbara F. Carneiro-Proietti, Juan Jonathan-Gonçalves, Ágata Lopes Ribeiro, Laurence Rodrigues do Amaral, Jordana Grazziela Coelho-dos-Reis, Matheus de Souza Gomes, Ana Lúcia Borges Starling, Giph
Rok vydání: 2017
Předmět:
0301 basic medicine
Time Factors
030106 microbiology
Immunology
Asymptomatic
Cell Line
Flow cytometry
Diagnosis
Differential
03 medical and health sciences
Myelopathy
Predictive Value of Tests
immune system diseases
Tropical spastic paraparesis
medicine
Cluster Analysis
Data Mining
Humans
Immunology and Allergy
Fluorescent Antibody Technique
Indirect
Human T-lymphotropic virus 1
medicine.diagnostic_test
Deltaretrovirus Antibodies
business.industry
Decision Trees
Reproducibility of Results
virus diseases
Viral Load
Flow Cytometry
medicine.disease
HTLV-I Infections
Paraparesis
Tropical Spastic
High-Throughput Screening Assays
Human T cell leukemia virus
Serum cytokine
030104 developmental biology
Case-Control Studies
Immunoglobulin G
Clinical diagnosis
Asymptomatic Diseases
Disease Progression
Cytokines
Differential diagnosis
medicine.symptom
business
Algorithms
Biomarkers
Zdroj: Journal of Immunological Methods. 444:36-46
ISSN: 0022-1759
Popis: The present work provides an innovative methodological approach to assess the anti-HTLV-1 IgG1 reactivity with practical application in clinical laboratory. Serum from non-infected healthy controls (NI) and HTLV-1-infected patients, categorized as asymptomatic (AS), putatively progressing to HTLV-1 associated myelopathy/tropical spastic paraparesis - HAM/TSP (pHAM) or with clinical diagnosis of HAM/TSP (HT) were assayed in two-parallel flow cytometry platforms, referred as: Fix and FixPerm protocols. Operating-characteristics analysis indicated that a single pair of attributes ("serum dilution/cut-off") for Fix and FixPerm protocols presented excellent performance for the diagnosis of HTLV-1 infection. Conversely, Fix and FixPerm protocols displayed weak/moderate overall performances when applied with prognosis purposes of HTLV-1 infection. A panoramic snapshot provided by the reactivity boards revealed clearly the higher sensitivity of FixPerm protocol for detecting seropositivity for HT, suggesting that stepwise combinatory criteria would improve the global performance of using a single pair of attributes. Three data mining strategies were tested, including endpoint titer analysis, heatmap assemblage and decision tree analysis. Bi-dimensional heatmap analysis demonstrated that, while the clustering profile of NI vs HTLV-1
Databáze: OpenAIRE
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