Genome editing of the SfABCC2 gene confers resistance to Cry1F toxin from Bacillus thuringiensis in Spodoptera frugiperda
| Autor: | Jin Minghui, Qi Li, Xiao-xu Sun, Jia-hui Tao, Kong-ming Wu, Cheng Ying, Yutao Xiao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
viruses Cry1F Agriculture (General) ABCC2 Plant Science Spodoptera medicine.disease_cause 01 natural sciences Biochemistry S1-972 Bt receptor Food Animals Genome editing Bacillus thuringiensis medicine CRISPR Gene CRISPR/Cas9 Genetics Ecology biology Toxin Strain (biology) fungi Spodoptera frugiperda 04 agricultural and veterinary sciences biology.organism_classification ABCC2 Gene 040103 agronomy & agriculture 0401 agriculture forestry and fisheries Animal Science and Zoology Agronomy and Crop Science 010606 plant biology & botany Food Science |
| Zdroj: | Journal of Integrative Agriculture, Vol 20, Iss 3, Pp 815-820 (2021) |
| ISSN: | 2095-3119 |
| Popis: | ATP-binding cassette transporter C2 (ABCC2) is known to be a receptor for Bacillus thuringiensis (Bt) toxins in several lepidopteran insects. Mutations in the ABCC2 gene have been genetically linked to field-evolved resistance to the Cry1F toxin from Bt in Spodoptera frugiperda. Here we generated a SfABCC2 knockout strain of S. frugiperda using the CRISPR/Cas9 system to provide further functional evidence of the role of this gene in susceptibility and resistance to Cry1F. Results from bioassays showed that the SfABCC2 knockout S. frugiperda strain displayed 118-fold resistance to Cry1F compared with the parental DH19 strain, but no resistance to Vip3A toxin from Bt. These results provide the first reverse genetic evidence for SfABCC2 as a functional receptor for Cry1F. |
| Databáze: | OpenAIRE |
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