Molecular evolution of mammalian lactate dehydrogenase-A genes and pseudogenes: association of a mouse processed pseudogene with a B1 repetitive sequence
| Autor: | Chi-Cheng Luo, S S.-L. Li, K. M. Fukasawa, K. Yagi, Wen-Hsiung Li |
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| Rok vydání: | 1986 |
| Předmět: |
Nonsynonymous substitution
Lactate dehydrogenase A Pseudogene Molecular Sequence Data Biology medicine.disease_cause Mice Molecular evolution Genetics medicine Animals Humans education Molecular Biology Gene Phylogeny Ecology Evolution Behavior and Systematics Repetitive Sequences Nucleic Acid education.field_of_study Mutation Base Sequence L-Lactate Dehydrogenase Phylogenetic tree Nucleic acid sequence Biological Evolution Molecular biology Pseudogenes |
| Zdroj: | Molecular Biology and Evolution. |
| ISSN: | 1537-1719 |
| Popis: | A mouse genomic clone containing a lactate dehydrogenase-A (LDH-A) processed pseudogene and a B1 repetitive element was isolated, and a nucleotide sequence of approximately 3 kb was determined. The pseudogene and B1 element are flanked by perfect 13-bp repeats, and the B1 sequence starts at 14 nucleotides 3' to the presumptive polyadenylation signal of the pseudogene. The nucleotide sequences of the LDH-A genes and processed pseudogenes from mouse, rat, and human were compared, and a phylogenetic tree was constructed. The rate and pattern of nucleotide substitutions in the LDH-A pseudogenes are similar to previously reported results (Li et al. 1984). The average rate of nucleotide substitutions in the LDH-A pseudogenes is 4.3 X 10(-9)/site/year. The substitutions of C----T and G----A are most frequent, and A----G substitutions are relatively high. The rate of synonymous substitutions in the LDH-A genes is 5.3 X 10(-9), which is not significantly higher than the average rate of 4.7 X 10(-9) for 35 mammalian genes. The rate of nonsynonymous substitutions in the LDH-A genes is 0.20 X 10(-9), which is considerably lower than the average rate of 0.88 X 10(-9) for 35 mammalian genes. Thus, the mammalian LDH-A gene appears to be highly conserved in evolution. |
| Databáze: | OpenAIRE |
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