Effect of thiamine pyrophosphate on retinopathy induced by hyperglycemia in rats: A biochemical and pathological evaluation
| Autor: | Emine Cinici, Durdu Altuner, Halis Suleyman, Osman Kukula, Nihal Cetin, Bahadir Suleyman, Ibrahim Ahiskali, Mehmet Kuzucu, Abdulkadir Coban, Hilal Balta |
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| Přispěvatelé: | EBYÜ, Tıp Fakültesi, OMÜ |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
Glutathione reductase 030226 pharmacology & pharmacy chemistry.chemical_compound 0302 clinical medicine lcsh:Ophthalmology Malondialdehyde Alloxan Edema rat Chromatography High Pressure Liquid oxidants Diabetic retinopathy Glutathione diabetic retinopathy antioxidants Biochemistry Vitamin B Complex Original Article Thiamine medicine.symptom Thiamine pyrophosphate Retinopathy medicine.medical_specialty Retina thiamine 03 medical and health sciences Retinal Diseases Internal medicine medicine Animals Rats Wistar Superoxide Dismutase business.industry medicine.disease Rats Disease Models Animal Oxidative Stress Ophthalmology Endocrinology chemistry lcsh:RE1-994 Hyperglycemia 030221 ophthalmology & optometry Thiamine Pyrophosphate business Biomarkers Follow-Up Studies |
| Zdroj: | Indian Journal of Ophthalmology, Vol 64, Iss 6, Pp 434-439 (2016) Indian Journal of Ophthalmology |
| Popis: | WOS: 000384321600005 PubMed: 27488151 Purpose: Information is lacking on the protective effects of thiamine pyrophosphate (TPP) against hyperglycemia-induced retinopathy in rats. This study investigated the biochemical and histopathological aspects of the effect of TPP on hyperglycemia-induced retinopathy induced by alloxan in rats. Materials and Methods: The rats were separated into a diabetic TPP-administered group (DTPG), a diabetes control group (DCG) and a healthy group (HG). While the DTPG was given TPP, the DCG and HG were administered distilled water as a solvent at the same concentrations. This procedure was repeated daily for 3 months. At the end of this period, all of the rats were euthanized under thiopental sodium anesthesia, and biochemical and histopathological analyses of the ocular retinal tissues were performed. The results of the DTPG were compared with those of the DCG and HG. Results: TPP prevented hyperglycemia by increasing the amount of malondialdehyde and decreasing endogen antioxidants, including total glutathione, glutathione reductase, glutathione S-transferase and superoxide dismutase. In addition, the amounts of the DNA oxidation product 8-hydroxyguanine were significantly lower in the retinas of the DTPG compared to the DCG. In the retinas of the DCG, there was a marked increase in vascular structures and congestion, in addition to edema. In contrast, little vascularization and edema were observed in the DTPG, and there was no congestion. The results suggest that TPP significantly reduced the degree of hyperglycemia-induced retinopathy. Conclusions: The results of this study indicate that TPP may be useful for prophylaxis against diabetic retinopathy. Erzincan University Scientific Research Projects Centre This study received the financial support from Erzincan University Scientific Research Projects Centre. |
| Databáze: | OpenAIRE |
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