Alterations in Adhesion, Transport, and Membrane Characteristics in an Adhesion-Deficient Pseudomonad
| Autor: | Mary F. DeFlaun, S. R. Oppenheimer, Madilyn Fletcher, Charles W. Condee, Sheryl H. Streger |
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| Rok vydání: | 1999 |
| Předmět: | |
| Zdroj: | Applied and Environmental Microbiology. 65:759-765 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.65.2.759-765.1999 |
| Popis: | A stable adhesion-deficient mutant of Burkholderia cepacia G4, a soil pseudomonad, was selected in a sand column assay. This mutant (ENV435) was compared to the wild-type strain by examining the adhesion of the organisms to silica sand and their transport through two aquifer sediments that differed in their sand, silt, and clay contents. We compared the longitudinal transport of the wild type and the adhesion mutant to the transport of a conservative chloride tracer in 25-cm-long glass columns. The transport of the wild-type strain was severely retarded compared to the transport of the conservative tracer in a variety of aquifer sediments, while the adhesion mutant and the conservative tracer traveled at similar rates. An intact sediment core study produced similar results; ENV435 was transported at a faster rate and in much greater numbers than G4. The results of hydrophobic interaction chromatography revealed that G4 was significantly more hydrophobic than ENV435, and polyacrylamide gel electrophoresis revealed significant differences in the lipopolysaccharide O-antigens of the adhesion mutant and the wild type. Differences in this cell surface polymer may explain the decreased adhesion of strain ENV435. |
| Databáze: | OpenAIRE |
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