Efficient laboratory evolution of computationally designed enzymes with low starting activities using fluorescence-activated droplet sorting

Autor:	Moritz Pott, Andrew D. Griffiths, Donald Hilvert, Cathleen Zeymer, Richard Obexer
Rok vydání:	2016
Předmět:	0301 basic medicine Computational enzyme design Directed evolution Fluorescence-activated droplet sorting Microfluidics Retro-aldolase Computer science Nanotechnology Bioengineering 010402 general chemistry 01 natural sciences Biochemistry 03 medical and health sciences Microtiter plate Fructose-Bisphosphate Aldolase Enzyme kinetics Amino Acid Sequence Throughput (business) Molecular Biology chemistry.chemical_classification biology 010405 organic chemistry Sorting Active site Flow Cytometry Fluorescence 0104 chemical sciences 030104 developmental biology Enzyme chemistry Mutation biology.protein Biophysics Computer-Aided Design Directed Molecular Evolution Biological system Biotechnology
Zdroj:	Protein Engineering, Design & Selection, 29 (9) Protein Engineering Design and Selection
ISSN:	1741-0134 1741-0126 0269-2139 1460-213X
Popis:	Protein Engineering, Design & Selection, 29 (9) ISSN:1741-0126 ISSN:1741-0134 ISSN:0269-2139 ISSN:1460-213X
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ca32bcaff562242d8f856a1e8e2da034 https://pubmed.ncbi.nlm.nih.gov/27542390 Zobrazit plný text záznamu