Influence of Different Glycoproteins and of the Virion Core on SERINC5 Antiviral Activity
| Autor: | Massimo Pizzato, Pyae Phyo Kyawe, Mehmet Hakan Guney, Jeremy Luban, Judith M. White, Teresa Vanzo, William E. Diehl |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
gag
retroviruses viruses restriction factor Microbiology Article Virus Equine infectious anemia 03 medical and health sciences Viral Envelope Proteins Viral entry Virology SERINC5 Murine leukemia virus Humans glycoproteins 030304 developmental biology 0303 health sciences Arenavirus virion biology pseudotype 030302 biochemistry & molecular biology Membrane Proteins virus diseases Virus Internalization biochemical phenomena metabolism and nutrition biology.organism_classification QR1-502 Leukemia Virus Murine HEK293 Cells Retroviridae Infectious Diseases Virion assembly Vesicular stomatitis virus Host-Pathogen Interactions Viruses HIV-1 Pseudotyping |
| Zdroj: | Viruses Volume 13 Issue 7 Viruses, Vol 13, Iss 1279, p 1279 (2021) |
| ISSN: | 1999-4915 |
| DOI: | 10.3390/v13071279 |
| Popis: | Host plasma membrane protein SERINC5 is incorporated into budding retrovirus particles where it blocks subsequent entry into susceptible target cells. Three structurally unrelated proteins encoded by diverse retroviruses, human immunodeficiency virus type 1 (HIV-1) Nef, equine infectious anemia virus (EIAV) S2, and ecotropic murine leukemia virus (MLV) GlycoGag, disrupt SERINC5 antiviral activity by redirecting SERINC5 from the site of virion assembly on the plasma membrane to an internal RAB7+ endosomal compartment. Pseudotyping retroviruses with particular glycoproteins, e.g., vesicular stomatitis virus glycoprotein (VSV G), renders the infectivity of particles resistant to inhibition by virion-associated SERINC5. To better understand viral determinants for SERINC5-sensitivity, the effect of SERINC5 was assessed using HIV-1, MLV, and Mason-Pfizer monkey virus (M-PMV) virion cores, pseudotyped with glycoproteins from Arenavirus, Coronavirus, Filovirus, Rhabdovirus, Paramyxovirus, and Orthomyxovirus genera. SERINC5 restricted virions pseudotyped with glycoproteins from several retroviruses, an orthomyxovirus, a rhabdovirus, a paramyxovirus, and an arenavirus. Infectivity of particles pseudotyped with HIV-1, amphotropic-MLV (A-MLV), or influenza A virus (IAV) glycoproteins, was decreased by SERINC5, whether the core was provided by HIV-1, MLV, or M-PMV. In contrast, particles pseudotyped with glycoproteins from M-PMV, parainfluenza virus 5 (PIV5), or rabies virus (RABV) were sensitive to SERINC5, but only with particular retroviral cores. Resistance to SERINC5 did not correlate with reduced SERINC5 incorporation into particles, route of viral entry, or absolute infectivity of the pseudotyped virions. These findings indicate that some non-retroviruses may be sensitive to SERINC5 and that, in addition to the viral glycoprotein, the retroviral core influences sensitivity to SERINC5. |
| Databáze: | OpenAIRE |
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