Bone phenotype of the aromatase deficient mouse
| Autor: | E. Tsyganov, Orhan K. Öz, Lydia Nanu, Gen Hirasawa, Peter P. Antich, Anca Constantinescu, Joseph E. Zerwekh, Jonathan N. Lawson, Ralph P. Mason, Robert W. Parkey, Evan R. Simpson |
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| Rok vydání: | 2002 |
| Předmět: |
Male
medicine.medical_specialty medicine.drug_class Endocrinology Diabetes and Metabolism Clinical Biochemistry Bone Marrow Cells Biochemistry Bone and Bones Mice Endocrinology Aromatase Bone Density Internal medicine medicine Animals Femur Insulin-Like Growth Factor I Molecular Biology Dual-energy X-ray absorptiometry Bone growth Bone mineral Mice Knockout Bone Development medicine.diagnostic_test biology Estradiol business.industry Cell Biology medicine.disease Magnetic Resonance Imaging Mice Inbred C57BL medicine.anatomical_structure Phenotype Animals Newborn Estrogen biology.protein Molecular Medicine Female Bone marrow Bone Remodeling Aromatase deficiency business Cancellous bone Tomography Emission-Computed |
| Zdroj: | The Journal of steroid biochemistry and molecular biology. 79(1-5) |
| ISSN: | 0960-0760 |
| Popis: | Estrogens are important for normal bone growth and metabolism. The mechanisms are incompletely understood. Thus, we have undertaken characterization of the skeletal phenotype of aromatase (ArKO) deficient mice. No abnormalities have been noted in skeletal patterning in newborns. Adult ArKO mice show decreased femur length and decreased peak Bone Mineral Density (BMD) with accelerated bone loss by 7 months of age in females. Magnetic resonance microscopy (MR) and microCT (microCT) imaging disclosed decreased cancellous connectivity and reduced cancellous bone volume in ArKO females. Bone formation rate (BFR) is increased in ArKO females and decreased in ArKO males. Estradiol therapy reverses these changes. This anabolic effect of estradiol in the male skeleton is supported by 18-F- Positron Emission Tomography (PET) imaging, which clearly demonstrates decreased spinal uptake, but marked increase after estradiol therapy. Serum IGF-1 levels are high in young female ArKO mice but low in young ArKO males. The reduced BMD in ArKO females, despite the presence of elevated serum IGF 1, suggests that other mechanism(s) are operative. There is increased B-cell lymphopoiesis in adult female ArKO bone marrow cells. These results show that ArKO mice show the effects of estrogen deficiency on bone growth, mass, metabolism, microarchitecture and the hematopoietic microenvironment. |
| Databáze: | OpenAIRE |
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