Expression of cell adhesion molecules in an established and characterized new human renal cell cancer line, CCF-RC7
| Autor: | Andrew C. Novick, James T. McMahon, Frank Steinbach, Kazunari Tanabe, Raymond R. Tubbs, R. Troy, Jeannine Alexander, Mark Edinger, Eric A. Klein |
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| Rok vydání: | 1995 |
| Předmět: |
Adult
Male Cell division Urology medicine.medical_treatment Mice Nude Mice Renal cell carcinoma medicine Doubling time Animals Humans Carcinoma Renal Cell Ploidies biology Cell adhesion molecule CD44 DNA Neoplasm medicine.disease Molecular biology Immunohistochemistry Kidney Neoplasms Cell biology Kinetics Cytokine Cell culture Karyotyping biology.protein Cytokines Female Cell Adhesion Molecules Clear cell Cell Division Neoplasm Transplantation |
| Zdroj: | Urological research. 23(3) |
| ISSN: | 0300-5623 |
| Popis: | In order to investigate the importance of cell adhesion molecules (CAMs) in renal cell carcinoma (RCC), a cell line, designated as CCF-RC7, was established from a human RCC of the clear cell type. CCF-RC7 was passaged over 50 times in vitro for 3 1/2 years. The cell line has an epithelial morphology and a doubling time of 30 h, forming colonies in soft agar with an average efficiency of 10.4% and producing clear cell tumors in athymic nude mice. CCF-RC7 cells have an aneuploid-hypotetraploid karyotype with a modal chromosome number of 82 and rearrangements in chromosomes 9, 12 and 14. Immunohistochemical and flow immunocytometric analyses revealed high expression of ICAM-1 (CD54), and Hermes antigen (CD44), which was significantly upregulated by cytokine and PMA treatment. VLA-4 was expressed on approximately 20% of tumor cells and could not be altered by cytokine or PMA stimulation. High expression of sialyl Lewis X was also demonstrated by immunohistological examination. This newly characterized cell line will serve as a useful model for the study of CAMs during hematogenous metastasis and host defense mechanisms in human RCC. |
| Databáze: | OpenAIRE |
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