Antibody repertoire development in fetal and neonatal piglets. XXIV. Hypothesis: The ileal Peyer patches (IPP) are the major source of primary, undiversified IgA antibodies in newborn piglets
| Autor: | David L. Francis, Marek Sinkora, Kristina Santiago-Mateo, Xiu-Zhu Sun, Nancy Wertz, John E. Butler |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Immunoglobulin A Swine Immunology Lumen (anatomy) Lymphocyte Activation Microbiology law.invention 03 medical and health sciences Probiotic Peyer's Patches 0302 clinical medicine Antibody Repertoire law Ileum Animals Germ-Free Life Cells Cultured Cell Proliferation Fetus B-Lymphocytes biology Probiotics Cell Differentiation Isotype Immunoglobulin Class Switching Gastrointestinal Microbiome Peyer Patch 030104 developmental biology Animals Newborn Immunoglobulin M Immunoglobulin A Secretory biology.protein Antibody Immunologic Memory 030215 immunology Developmental Biology Antibody Diversity |
| Zdroj: | Developmental and comparative immunology. 65 |
| ISSN: | 1879-0089 |
| Popis: | The ileal Peyers patches (IPP) of newborn germfree (GF) piglets were isolated into blind loops and the piglets colonized with a defined probiotic microflora. After 5 weeks, IgA levels in the intestinal lavage (IL) of loop piglets remained at GF levels and IgM comprised ∼70% while in controls, IgA levels were elevated 5-fold and comprised ∼70% of total Igs. Loop piglets also had reduced serum IgA levels suggesting the source of serum IgA had been interrupted. The isotype profile for loop contents was intermediate between that in the IL of GF and probiotic controls. Surprisingly, colonization alone did not result in repertoire diversification in the IPP. Rather, colonization promoted pronounced proliferation of fully switched IgA(+)IgM(-) B cells in the IPP that supply early, non-diversified "natural" SIgA antibodies to the gut lumen and a primary IgA response in serum. |
| Databáze: | OpenAIRE |
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