Critical Role of Prohibitin in Endothelial Cell Apoptosis Caused by Glycated Low-density Lipoproteins and Protective Effects of Grape Seed Procyanidin B2
Autor: | Jianhua Zhang, Fei Yu, Hai-Qing Gao, Qian Cai, Baoying Li, Wen-bin Yin, Jun-fu Wang, Zhen Zhang, Rui-Hai Zhou, Xiao-li Li, Mei Cheng |
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Rok vydání: | 2015 |
Předmět: |
Cell Survival
Apoptosis macromolecular substances Mitochondrion Biology Catechin Umbilical vein chemistry.chemical_compound Prohibitins Human Umbilical Vein Endothelial Cells medicine Biflavonoids Humans Proanthocyanidins Phosphorylation RNA Small Interfering Endothelial dysfunction Prohibitin Procyanidin B2 Pharmacology Grape Seed Extract Cytochrome c technology industry and agriculture medicine.disease Mitochondria Cell biology Lipoproteins LDL Repressor Proteins Cytosol Gene Expression Regulation chemistry Biochemistry biology.protein lipids (amino acids peptides and proteins) Cardiology and Cardiovascular Medicine |
Zdroj: | Journal of Cardiovascular Pharmacology. 65:13-21 |
ISSN: | 0160-2446 |
Popis: | Elevated level of glycated low-density lipoproteins (glyLDL) is believed to contribute to endothelial dysfunction, which is involved in the pathogenesis and acceleration of diabetic vascular diseases. Grape seed procyanidin B2 (GSPB2) has been reported to possess protective effects against endothelial dysfunction. However, the underlying mechanism remains unclear. Prohibitin (PHB) is a multifunctional protein implicated in cellular survival and apoptosis. In this study, we showed that glyLDL treatment decreased protein level of PHB, reduced viability, and increased apoptosis in human umbilical vein endothelial cells (HUVEC). PHB overexpression or GSPB2 significantly attenuated apoptosis induced by glyLDL. Moreover, PHB siRNA increased HUVEC apoptosis, along with defective mitochondria and increased levels of cytosol cytochrome c concentration, caspase-3 activity, Bax/Bcl-2 ratio, and phosphorylated Akt, whereas PHB overexpression or GSPB2 restored these changes. Our study identified PHB as an important player responsible for HUVEC apoptosis induced by glyLDL. GSPB2 protected against HUVEC apoptosis at least in part through upregulating PHB. Targeting PHB could be significant in fighting against diabetic vascular complications. |
Databáze: | OpenAIRE |
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