Characterization of ouabain resistant, hypoxanthine phosphoribosyl transferase deficient human cells and their usefulness as a general method for the production of human cell hybrids
| Autor: | B.E. Weissman, E.J. Stanbridge |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Genetic Markers
Hypoxanthine Phosphoribosyltransferase General method Mutant Drug Resistance Hybrid Cells Biology Ouabain Cell Line HeLa chemistry.chemical_compound Genetics medicine Chromosomes Human Humans Molecular Biology Genetics (clinical) Hypoxanthine Adenosine Triphosphatases Phosphoribosyl transferase Neoplasms Experimental Human cell biology.organism_classification Molecular biology Phenotype chemistry Biochemistry Ploidy HeLa Cells medicine.drug |
| Zdroj: | Cytogenetic and Genome Research. 28:227-239 |
| ISSN: | 1424-859X 1424-8581 |
| DOI: | 10.1159/000131536 |
| Popis: | Ouabain resistant mutants of hypoxanthine phosphoribosyl transferase deficient HeLa cells and euploid human fibroblasts were isolated and extensively characterized. These double mutants were used to test the prediction that they would be “universal hybridizers” for intraspecific human cell hybrid production. Hybrids were selected from fusions with human cells of diverse somatic origin. In addition it was shown that multi-parental hybrids can be generated using a modification of the selection system, a result which will be useful for gene dosage experiments involving human cells. |
| Databáze: | OpenAIRE |
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