Isolation and characterization of insulin-like growth factor-II from human bone
| Autor: | Lee F. Ellis, Charles A. Frolik, Daniel C. Williams |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Hydrochloride
medicine.medical_treatment Biophysics Biochemistry High-performance liquid chromatography Bone and Bones Cell Line chemistry.chemical_compound Insulin-Like Growth Factor II Leucine Somatomedins medicine Animals Humans Insulin-Like Growth Factor I Guanidine Molecular Biology Chromatography High Pressure Liquid Chromatography Growth factor Cell Biology Rats Molecular Weight chemistry Cell culture Transforming Growth Factors Chromatography Gel Thymidine Peptides Transforming growth factor |
| Zdroj: | Biochemical and biophysical research communications. 151(3) |
| ISSN: | 0006-291X |
| Popis: | Human bone was sequentially extracted with 4 M guanidine hydrochloride to remove nonmineralized tissue components, 0.5 M EDTA to dissolve the mineral phase, 4 M guanidine hydrochloride to remove matrix associated proteins and finally a combination of 4 M guanidine hydrochloride and 0.5 M EDTA to remove residual proteins. The extracts were examined for the presence of factors that were able to stimulate the incorporation of [3H] thymidine into DNA and [14C] leucine into protein in a cloned rat bone cell culture system. The majority of the bioactivity was found in the first guanidine hydrochloride extract (59 +/- 12%) while the second guandine hydrochloride extract contained 27 +/- 8%. In addition to several known growth factors already reported to be present in bone (transforming growth factor-beta and insulin-like growth factor-I) insulin-like growth factor-II was identified by its chromatographic, electrophoretic and immunological properties as well as by N-terminal sequence data. The insulin-like growth factor-II levels (802 +/- 112 micrograms/kg wet weight bone) were 10 fold higher than that found for insulin-like growth factor-I (84 +/- 23 micrograms/kg wet weight). |
| Databáze: | OpenAIRE |
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