Isolation and identification of phytoestrogens and flavonoids in an Ayurvedic proprietary medicine using chromatographic and Mass Spectroscopic analysis
| Autor: | Indira Balachandran, A Arun, C. T. Sulaiman, E. M. Anandan, CR Sandhya |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
endocrine system
Chromatography General Veterinary Traditional medicine lcsh:R Ms analysis Obstetrics and Gynecology food and beverages lcsh:Medicine Phytoestrogens LC/MS Plant Science Glycitein Coumestrol Isoflavones chemistry.chemical_compound Reproductive Medicine chemistry Liquid chromatography–mass spectrometry Animal Science and Zoology Herbal medicine |
| Zdroj: | Asian Pacific Journal of Reproduction, Vol 4, Iss 2, Pp 153-156 (2015) |
| ISSN: | 2305-0500 |
| Popis: | Objective To develop analytical methods for the isolation and structural identification of poly phenols including phytoestrogens in Mensokot tablet, a herbal proprietary medicine. Methods Isolation consisted of an ultrasound-assisted extraction, followed by acid hydrolysis and a final liquid-liquid extraction step in diethyl ether. Identification and structural characterisation was done by liquid chromatography coupled with Q-TOF-ESI-MS/MS analysis. Results Phytoestrogens such as Coumestrol, Genistein and Glycitein have been identified in Mensokot tablet along with several other flavonoids. Conclusions In the present research, a rapid HPLC-MS/MS method has been developed for the identification of phytoestrogens and other flavonoids from an Ayurvedic proprietary medicine. Phytoestrogens are considered to play an important role in the prevention of cancers, heart disease, menopausal symptoms and osteoporosis. |
| Databáze: | OpenAIRE |
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