NMR and ESR study of amphotericin B interactions with various binary phosphatidylcholine/phosphatidylglycerol membranes
| Autor: | Mohamed Skiba, Malika Lahiani-Skiba, J. C. Debouzy, D. Crouzier, G. Nugue, L. Mehenni |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Phosphatidylglycerol Liposome Magnetic Resonance Spectroscopy Chromatography 030106 microbiology Electron Spin Resonance Spectroscopy technology industry and agriculture Pharmaceutical Science Phosphatidylglycerols 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Membrane Solubility Pulmonary surfactant chemistry In vivo Solubilization Amphotericin B Phosphatidylcholine medicine Dimyristoylphosphatidylcholine medicine.drug |
| Zdroj: | International Journal of Pharmaceutics. 521:384-394 |
| ISSN: | 0378-5173 |
| DOI: | 10.1016/j.ijpharm.2017.02.034 |
| Popis: | Several biologically relevant phospholipids are considered as potential excipients for IV administration liposome’s formulation of AMB (Biopharmaceututics Classification System Class IV). On the basis of in vivo bioavaibility studies, DMPC and DMPG were ranked as the first potent encapsulation enhancers for this model drug, especially if one expects to target DMPG rich systems as pulmonary surfactant. Subsequently, dispersions (multilayers) of DMPC, DMPG or in binary systems with various molar ratios were prepared with or without AMB (molar ratios AMB/lipid) and further investigated using the 1 H-, 31 P-NMR methods. It was found that equimolar preparations of DMPG/DMPG exhibited both a good encapsulation of AMB, while also probably able to target pulmonary surfactant. Besides DMPG did not exhibit the same solubilization properties. Conversely, no targeting by DMPC dispersion alone was expected, even if a good solubilization was obtained. |
| Databáze: | OpenAIRE |
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