Plasmodium falciparum GPCR-like receptor SR25 mediates extracellular K+ sensing coupled to Ca2+ signaling and stress survival
| Autor: | Marta Ponzi, Tomasino Pace, Leonardo Picci, Miriam S. Moraes, Maneesh Kumar Singh, Chiara Currà, Célia R.S. Garcia, Lucas Borges-Pereira, Tullio Pozzan, Alexandre Budu, Julio Levano-Garcia |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
030231 tropical medicine lcsh:Medicine Parasitemia Parasite load Nitric oxide 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Extracellular medicine Receptor lcsh:Science Multidisciplinary biology Phospholipase C lcsh:R Wild type Plasmodium falciparum biology.organism_classification medicine.disease Molecular biology 030104 developmental biology chemistry Immunology lcsh:Q |
| Zdroj: | Scientific Reports, Vol 7, Iss 1, Pp 1-13 (2017) |
| ISSN: | 2045-2322 |
| Popis: | The malaria parasite Plasmodium falciparum is exposed, during its development, to major changes of ionic composition in its surrounding medium. We demonstrate that the P. falciparum serpentine-like receptor PfSR25 is a monovalent cation sensor capable of modulating Ca2+ signaling in the parasites. Changing from high (140 mM) to low (5.4 mM) KCl concentration triggers [Ca2+]cyt increase in isolated parasites and this Ca2+ rise is blocked either by phospholipase C (PLC) inhibition or by depleting the parasite’s internal Ca2+ pools. This response persists even in the absence of free extracellular Ca2+ and cannot be elicited by addition of Na+, Mg2+ or Ca2+. However, when the PfSR25 gene was deleted, no effect on [Ca2+]cyt was observed in response to changing KCl concentration in the knocked out (PfSR25−) parasite. Finally, we also demonstrate that: i) PfSR25 plays a role in parasite volume regulation, as hyperosmotic stress induces a significant decrease in parasite volume in wild type (wt), but not in PfSR25− parasites; ii) parasites lacking PfSR25 show decreased parasitemia and metacaspase gene expression on exposure to the nitric oxide donor sodium nitroprusside (SNP) and iii), compared to PfSR25− parasites, wt parasites showed a better survival in albumax-deprived condition. |
| Databáze: | OpenAIRE |
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