Molecular phylogeny of trematodes in Family Heterophyidae based on mitochondrial cytochrome c oxidase subunit I (mCOI)
| Autor: | Chalobol Wongsawad, Siriwadee Chomdej, Jong-Yil Chai, Thapana Chontananarth, Duangduen Krailas |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
food.ingredient
Zoology Heterophyidae Trematode Infections Phylogenetic relationship Electron Transport Complex IV Metagonimus food Genus parasitic diseases Animals Clade mCOI Phylogeny Medicine(all) biology Phylogenetic tree Fishes General Medicine Helminth Proteins DNA Helminth biology.organism_classification Haplorchis Sister group Evolutionary biology Molecular phylogenetics |
| Zdroj: | Asian Pacific journal of tropical medicine. 7(6) |
| ISSN: | 2352-4146 |
| Popis: | Objective To analyze a phylogenetic tree for understanding the molecular systematic of trematode in Family Heterophyidae, which are highly distributed in Thailand. Methods Based on thirteen sequences of mitochondrial cytochrome c oxidase subunit I (mCOI) gene from six genera of heterophyid trematodes, viz. Haplorchis, Stellantchasmus, Centrocestus, Metagonimus, Pygidopsis , and Haplorchoides were aligned automatically using the Clustal × 2.0 program. A phylogenetic tree was constructed by maximum likeinghood (ML) and neighbor-joining (NJ) methods, with 1 000 bootstrap using the 5.0 program. Results The phylogenetic relationship from both methods was similar and separated into three groups consisting of Haplorchoides pumilio group, Haplorchoides taichui group and another heterophyid genera. Conclusions The sequence data of mtCOI can be used to investigate the phylogenetic relationships of trematodes at the genus level. Each clade of different genera of heterophyid trematodes can be separated into sister groups that correlated with the morphological characteristic, kind of secondary intermediate host and geographic distribution. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|