| Autor: |
Jing, Ding, Yan, Zhang, Shanqi, Wan, Liu, Yang, Xiaohua, Cui, Caiping, Zhao, Suo, Zhang, Zhuocheng, Meng |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Clinical Biochemistry. :49-56 |
| ISSN: |
0009-9120 |
| Popis: |
This study aims to establish a novel method for measuring perospirone in human plasma for therapeutic drug monitoring (TDM) by liquid chromatography-mass spectrometry (LC-MS) coupled with an automatic liquid chromatograph mass spectrometer coupler 9500 (LC-MS/MS-Mate 9500), which has been equipped with self-internal standard (SIS) calibration technology.A novel and attractive analytical calibration method designed for perospirone, calibration with SIS, was reported. After protein precipitation with acetonitrile-cyclopentanol (9:1, v/v) containing 1% NHThis method showed good selectivity because no interfering peaks were observed in the plasma samples during the 2.0-min run time. The calibration curve range was 0.05-20 ng/mL, with a correlation coefficient of ≥ 0.9995. Intraday and interday accuracies were 98.3%-107.9%, respectively, with precision relative standard deviation values of 10%. The matrix effects ranged from 92.7% to 96.1%, and extraction recoveries were between 97.3% and 108.8%. Finally, this method was successfully applied to routine clinical TDM for 142 patients. The perospirone plasma concentrations of the patients ranged between 0.07 and 10.96 ng/mL.This bioanalytical method can be used for the quantification of perospirone in human plasma by LC-MS/MS-Mate 9500 using perospirone itself as the SIS. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect