Quantification of Ricinine and Abrine in Human Plasma by HPLC–MS-MS: Biomarkers of Exposure to Ricin and Abrin
| Autor: | Sean T Carlsen, Samantha L. Isenberg, Aleksandra I Noras, Melissa D. Carter, Michael A. Miller, Rudolph C. Johnson, Mike A. Mojica, Jerry D. Thomas, Chinthaka P Bulathsinghala |
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| Rok vydání: | 2018 |
| Předmět: |
Pyridones
Health Toxicology and Mutagenesis Ricin Urine Toxicology 01 natural sciences High-performance liquid chromatography Article Indole Alkaloids Specimen Handling Analytical Chemistry Forensic Toxicology 03 medical and health sciences chemistry.chemical_compound Alkaloids 0302 clinical medicine Limit of Detection Humans Environmental Chemistry Protein precipitation Sample preparation 030212 general & internal medicine Detection limit Chemical Health and Safety Chromatography Poisoning 010401 analytical chemistry Reproducibility of Results 0104 chemical sciences chemistry Calibration Abrin Sample collection Biomarkers |
| Zdroj: | Journal of Analytical Toxicology. |
| ISSN: | 1945-2403 0146-4760 |
| DOI: | 10.1093/jat/bky040 |
| Popis: | Ricin and abrin are toxic ribosome-inactivating proteins found in plants. Exposure to these toxins can be detected using the biomarkers ricinine and abrine, which are present in the same plant sources as the toxins. The concentration of the biomarkers in urine and blood will be dependent upon the purification of abrin or ricin, the route of exposure, and the length of time between exposure and sample collection. Here, we present the first diagnostic assay for the simultaneous quantification of both ricinine and abrine in blood matrices. Furthermore, this is the first-ever method that may detect abrine in blood products. Samples were processed by isotope-dilution, solid-phase extraction, protein precipitation, and quantification by HPLC-MS/MS. This analytical method detects abrine from 5.00 to 500 ng/mL and ricinine from 0.300 to 300 ng/mL with coefficients of determination of 0.996 ± 0.003 and 0.998 ± 0.002 (n=22), respectively. Quality control material accuracy was determined to have less than 10% relative error, and precision was within 19% relative standard deviation. The assay’s time-to-first result is three hours including sample preparation. Furthermore, the method was applied for the quantification of ricinine in the blood of a patient who had intentionally ingested castor beans to demonstrate the test was fit-for-purpose. This assay was designed to support the diagnosis of ricin and abrin exposures in public health investigations. |
| Databáze: | OpenAIRE |
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