Identifying membrane-bound quinoprotein glucose dehydrogenase from acetic acid bacteria that produce lactobionic and cellobionic acids
| Autor: | Hiromi Murakami, Shigemitsu Tanaka, Taro Kiso, Daisuke Koma, Takaaki Kiryu |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Glucose Dehydrogenases Lactose Disaccharides 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry Substrate Specificity 03 medical and health sciences chemistry.chemical_compound Acetic acid 010608 biotechnology Acetic acid bacteria Molecular Biology Quinoprotein glucose dehydrogenase chemistry.chemical_classification biology Organic Chemistry Cell Membrane General Medicine biology.organism_classification Amperometry Lactobionic acid 030104 developmental biology Enzyme Glucose chemistry Acetobacteraceae Oxidation-Reduction Bacteria Biotechnology |
| Zdroj: | Bioscience, biotechnology, and biochemistry. 83(6) |
| ISSN: | 1347-6947 |
| Popis: | Acetic acid bacteria are used in the commercial production of lactobionic acid (LacA). However, the lactose-oxidizing enzyme of these bacteria remains unidentified. Lactose-oxidizing activity has been detected in bacterial membrane fractions and is strongly inhibited by d-glucose, suggesting that the enzyme was a membrane-bound quinoprotein glucose dehydrogenase, but these dehydrogenases have been reported to be incapable of oxidizing lactose. Thus, we generated m-GDH-overexpressing and -deficient strains of Komagataeibacter medellinensis NBRC3288 and investigated their lactose-oxidizing activities. Whereas the overexpressing variants produced ~2–5-fold higher amounts of LacA than the wild-type strains, the deficient variant produced no LacA or d-gluconic acid. Our results indicate that the lactose-oxidizing enzyme from acetic acid bacteria is membrane-bound quinoprotein glucose dehydrogenase. Abbreviations: LacA: lactobionic acid; AAB: acetic acid bacterium; m-GDH: membrane-bound quinoprotein glucose dehydrogenase; DCIP: 2,6-dichlorophenolindophenol; HPAEC-PAD: high-performance anion-exchange chromatography with pulsed amperometric detection |
| Databáze: | OpenAIRE |
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