Phospholipid Content of Pseudomonas aeruginosa PAO1 Is Modulated by the Growth Phase Rather Than the Immobilization State
| Autor: | Sébastien Vilain, Corinne Buré, Patricia Costaglioli, Marc Crouzet, Christophe Barthe, Caroline Le Sénéchal |
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| Přispěvatelé: | Chimie et Biologie des Membranes et des Nanoobjets (CBMN), École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Phospholipid Attachment Bacterial growth medicine.disease_cause Biochemistry Incubation period 03 medical and health sciences chemistry.chemical_compound Tandem Mass Spectrometry Lipidomics medicine Incubation Phospholipids Degree of unsaturation 030109 nutrition & dietetics Mass spectrometry Pseudomonas aeruginosa Biofilm Organic Chemistry Cell Biology [CHIM.MATE]Chemical Sciences/Material chemistry 030104 developmental biology chemistry Chromatography Liquid |
| Zdroj: | Lipids Lipids, Springer Verlag, In press, 54 (9), pp.519-529. ⟨10.1002/lipd.12184⟩ |
| ISSN: | 0024-4201 1558-9307 |
| DOI: | 10.1002/lipd.12184⟩ |
| Popis: | Biofilms have significance in medical, industrial, and environmental settings, and can cause important damage. As biofilms are tolerant to various stresses, including antibiotics, it is necessary to better understand their formation. For this reason, we characterized the phospholipidome of Pseudomonas aeruginosa, an opportunistic pathogen involved in numerous infections, during the first steps of the biofilm development. By a liquid chromatography-tandem mass spectrometry time-course analysis over a 24-h period, we compared the phospholipid (PL) composition of immobilized (attached) and planktonic (unattached) P. aeruginosa PAO1 cells. Our results showed that the PL content of P. aeruginosa PAO1 was mainly modulated by the incubation time, thus related to bacterial growth but also, more modestly, by the immobilization state. We observed that relative amounts of PL varied over time with two main profiles and that these profiles are correlated to its fatty acid composition, including the degree of unsaturation. A statistical analysis revealed that the PL contents of both attached and unattached PAO1 cells were significantly different mainly after 3 and 6 h of incubation and that the amounts of two PL presented a statistical difference between attached and unattached cells all along the 24-h period: PtdEtn 16:0_18:1 and PtdEtn 18:1_18:1. |
| Databáze: | OpenAIRE |
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