Genetic, phenotypic and matrix-assisted laser desorption ionization time-of-flight mass spectrometry-based identification of anaerobic bacteria and determination of their antimicrobial susceptibility at a University Hospital in Japan
| Autor: | Taro Noguchi, Yasufumi Matsumura, Karin Kato, Tomoyuki Yunoki, Satoshi Nakano, Masaki Yamamoto, Shunji Takakura, Satoshi Ichiyama, Miki Nagao, Go Hotta |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) 030106 microbiology Microbial Sensitivity Tests Mass spectrometry Microbiology Cohort Studies Hospitals University Bacteria Anaerobic 03 medical and health sciences Clostridium Japan medicine Prevotella Humans Pharmacology (medical) biology Bacterial Infections Clostridium difficile biology.organism_classification Anti-Bacterial Agents Bacterial Typing Techniques Metronidazole Matrix-assisted laser desorption/ionization 030104 developmental biology Infectious Diseases Female Anaerobic bacteria Bacteroides medicine.drug |
| Zdroj: | Journal of Infection and Chemotherapy. 22:303-307 |
| ISSN: | 1341-321X |
| DOI: | 10.1016/j.jiac.2016.01.014 |
| Popis: | The accuracies of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and the phenotypic method using VITEK 2 were compared to the accuracy of 16S rRNA sequence analysis for the identification of 170 clinically isolated anaerobes. The antimicrobial susceptibility of the isolates was also evaluated. Genetic analysis identified 21 Gram-positive species in 14 genera and 29 Gram-negative species in 11 genera. The most frequently isolated genera were Prevotella spp. (n = 46), Bacteroides spp. (n = 25) and Clostridium spp. (n = 25). MALDI-TOF MS correctly identified more isolates compared with VITEK 2 at the species (80 vs. 58%, respectively; p |
| Databáze: | OpenAIRE |
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