Vat, an Amazing Gene Conferring Resistance to Aphids and Viruses They Carry: From Molecular Structure to Field Effects
| Autor: | Flavie Vanlerberghe-Masutti, Nathalie Boissot, Alexandra Schoeny |
|---|---|
| Přispěvatelé: | Unité de recherche Génétique et amélioration des fruits et légumes (GALF), Institut National de la Recherche Agronomique (INRA), Station de Pathologie Végétale (AVI-PATHO), Centre de Biologie pour la Gestion des Populations (UMR CBGP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Génétique et Amélioration des Fruits et Légumes (GAFL), Unité de Pathologie Végétale (PV), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Boissot, Nathalie |
| Rok vydání: | 2016 |
| Předmět: |
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences
0106 biological sciences 0301 basic medicine genetic control résistance aux insectes Virologie Review Plant Science culture légumière de plein champ 01 natural sciences Resistance to pests revue de synthèse chemistry.chemical_compound NLR resistance gene Aphis gossypii melon Plant defense against herbivory pathologie végétale vecteur de virus 2. Zero hunger Aphid Vegetal Biology mode d'action biologique Effector resistance to insects food and beverages durability cucumis melo aphis gossypii resistance deployment resistance to viruses gène de résistance gène vat Agricultural sciences plant pathogen interaction Cucumis Phytopathology and phytopharmacy lcsh:Plant culture Biology résistance des plantes aux virus Crop 03 medical and health sciences resistance gene phytopathogenic virus Virology parasitic diseases Botany [SDV.BV]Life Sciences [q-bio]/Vegetal Biology lcsh:SB1-1110 lutte génétique Gene virus phytopathogène interaction plante pathogène Callose nutritional and metabolic diseases biochemical phenomena metabolism and nutrition biology.organism_classification Phytopathologie et phytopharmacie 030104 developmental biology chemistry human activities Biologie végétale Sciences agricoles 010606 plant biology & botany |
| Zdroj: | Frontiers in Plant Science Frontiers in Plant Science, Frontiers, 2016, 7:1420, pp.1-18. ⟨10.3389/fpls.2016.01420⟩ Frontiers in Plant Science, Vol 7 (2016) Frontiers in Plant Science (7:1420), 1-18. (2016) Frontiers in Plant Science, 2016, 7, pp.1420. ⟨10.3389/fpls.2016.01420⟩ |
| ISSN: | 1664-462X |
| DOI: | 10.3389/fpls.2016.01420 |
| Popis: | We review half a century of research on Cucumis melo resistance to Aphis gossypii from molecular to field levels. The Vat gene is unique in conferring resistance to both A. gossypii and the viruses it transmits. This double phenotype is aphid clone-dependent and has been observed in 25 melon accessions, mostly from Asia. It is controlled by a cluster of genes including CC-NLR, which has been characterized in detail. Copy-number polymorphisms (for the whole gene and for a domain that stands out in the LLR region) and single-nucleotide polymorphisms have been identified in the Vat cluster. The role of these polymorphisms in plant aphid/interactions remains unclear. The Vat gene structure suggests a functioning with separate recognition and response phases. During the recognition phase, the VAT protein is thought to interact (likely indirectly) with an aphid effector introduced during cell puncture by the aphid. A few hours later, several miRNAs are upregulated in Vat plants. Peroxidase activity increases, and callose and lignin are deposited in the walls of the cells adjacent to the stylet path, disturbing aphid behavior. In aphids feeding on Vat plants, Piwi-interacting RNA-like sequences are abundant and the levels of other miRNAs are modified. At the plant level, resistance to aphids is quantitative (aphids escape the plant and display low rates of reproduction). Resistance to viruses is qualitative and local.Durability of NLR genes is highly variable. A. gossypii clones are adapted to Vat resistance, either by introducing a new effector that interferes with the deployment of plant defenses, or by adapting to the defenses it triggered. Viruses transmitted in a non-persistent manner cannot adapt to Vat resistance. At population level, Vat reduces aphid density and genetic diversity. The durability of Vat resistance to A. gossypii populations depends strongly on the agro-ecosystem, including, in particular, the presence of other cucurbit crops serving as alternative hosts for adapted clones in fall and winter. At the crop level, Vat resistance decreases the intensity of Cucumber Mosaic Virus (CMV) and Cucurbit aphid-borne yellows virus (CABYV) epidemics. The durability of resistance to CABYV could potentially be increased if combined with Vat resistance. |
| Databáze: | OpenAIRE |
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