A Real Time PCR strategy for the detection and quantification of Candida albicans in human blood
| Autor: | Gilda Maria Barbaro Del Negro, Maria Aparecida Shikanai-Yasuda, Vera Lúcia Teixeira de Freitas, Marta Heloísa Lopes, Vivian Caso Coelho, Claudia de Abreu Fonseca, Felipe Delatorre Busser, Marcello Magri |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
RC955-962
030231 tropical medicine ITS2 Real-Time Polymerase Chain Reaction Brief Communication Sensitivity and Specificity Microbiology 03 medical and health sciences Quantitative PCR 0302 clinical medicine Arctic medicine. Tropical medicine DNA Ribosomal Spacer Candida albicans Humans DNA Fungal Detection limit biology Human blood Fungal genetics Reproducibility of Results Candidemia biology.organism_classification Corpus albicans Yeast Standard curve Invasive candidiasis Detection Real-time polymerase chain reaction |
| Zdroj: | Revista do Instituto de Medicina Tropical de São Paulo; Vol. 62 (2020); e9 Revista do Instituto de Medicina Tropical de São Paulo; v. 62 (2020); e9 Revista do Instituto de Medicina Tropical de São Paulo Instituto de Medicina Tropical (IMT) instacron:IMT Revista do Instituto de Medicina Tropical de São Paulo, Vol 62 (2020) Revista do Instituto de Medicina Tropical de São Paulo, Volume: 62, Article number: e9, Published: 07 FEB 2020 |
| ISSN: | 1678-9946 0036-4665 |
| Popis: | Candidemia is a significant cause of bloodstream infections (BSI) in nosocomial settings. The identification of species can potentially improve the quality of care and decrease human mortality. Quantitative PCR (qPCR) was evaluated for Candida albicans detection using culture suspensions containing C. albicans , spiked human blood, the cloned qPCR target fragment (ITS2 region) and the results of these assays were compared. The assays showed a good detection limit: C. albicans DNA extracted from yeast (sensitivity 0.2 CFU/µL), spiked human blood (sensitivity 10 CFU/mL), and cloned fragment of ITS2 region (sensitivity 20 target copies/μL). The efficiency of ITS2 fragment-qPCR ranged from 89.67 to 97.07, and the linearity (R2) of the standard curve ranged from 0.992 to 0.999. The results showed that this ITS2-qPCR has a great potential as a molecular prototype model for the development of a test to be applied in clinical practice, greatly reducing the time of candidemia diagnosis, which is extremely important in this clinical setting. |
| Databáze: | OpenAIRE |
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