Effects of ozone preconditioning on recovery of rat colon anastomosis after preoperative radiotherapy
| Autor: | Gülin Ergun Taşdöven, Fatma Ayca Gultekin, Ilhan Tasdoven, Muzaffer Onder Oner, Banu Dogan Gun, Ummuhani Ozel Turkcu, Ali Ugur Emre, Bekir Hakan Bakkal |
|---|---|
| Přispěvatelé: | MÜ |
| Rok vydání: | 2020 |
| Předmět: |
030213 general clinical medicine
medicine.medical_specialty Antioxidant Colorectal cancer Colon medicine.medical_treatment Urology Medicine (miscellaneous) Anastomosis General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound Hydroxyproline 0302 clinical medicine Ozone Malondialdehyde Preoperative Care Internal Medicine medicine Animals Pharmacology (medical) Rats Wistar Saline Genetics (clinical) Wound Healing Radiotherapy biology business.industry Anastomosis Surgical medicine.disease Pathophysiology Rats Anastomotic Leakage Colon Anastomosis chemistry Myeloperoxidase Reviews and References (medical) biology.protein Radiotherapy Adjuvant Ozone Oxidative Preconditioning business |
| Zdroj: | Advances in clinical and experimental medicine : official organ Wroclaw Medical University. 28(12) |
| ISSN: | 1899-5276 |
| Popis: | Gultekin, Fatma Ayca/0000-0002-4148-5871 WOS: 000505207100011 PubMed ID: 31895975 Background. Anastomotic leakage is a devastating complication of colorectal surgery. Neoadjuvant radiotherapy for colorectal cancer can affect the mechanical and biochemical parameters of anastomotic healing. It has been reported that ozone increases antioxidant enzyme activity and stimulates adaptive processes to oppose the pathophysiologic conditions mediated by reactive oxygen species (ROS). Objectives. The objective of this study was to investigate the effect of controlled administration of ozone on the healing of anastomosis and the activation of antioxidant enzymes in the colon after radiotherapy. Material and methods. Rats (n = 48) were randomly assigned to the following groups: control groups (1 and 2), saline-treated and irradiated (IR) groups (3 and 4) and ozone oxidative preconditioning (OOP) and IR groups (5 and 6). Rats were exposed to whole-body IR (6 Gy) after pretreatment with either saline or ozone. Rats in groups 1, 3 and 5 were euthanized on postoperative day 3, whereas those in groups 2, 4 and 6 were euthanized on postoperative day 7. The anastomoses were performed on day 7 post-IR. The anastomotic segment was resected to measure hydroxyproline (HPO) content, myeloperoxidase (MPO) activity and malondialdehyde (MDA) concentration and for histopathological evaluation. Results. The mean bursting pressure of the groups that underwent radiotherapy was lower than that of the control groups (p < 0.001). In groups 5 and 6, the tissue HPO concentrations were higher than those in groups 3 and 4. Although mean values for MPO activity in groups Sand 6 were higherthan those in groups 3 and 4, the differences were not significant. Regarding oxidative damage markers, MDA concentrations were significantly lower in group 5 than those in group 3. Conclusions. In this experimental model, OOP exerted favorable effects on colon anastomotic healing after radiation exposure. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|