Evaluation of different culture media to support in vitro growth and biofilm formation of bacterial vaginosis-associated anaerobes
| Autor: | A. Rosca, Nuno Cerca, Joana Castro |
|---|---|
| Přispěvatelé: | Universidade do Minho |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
ved/biology.organism_classification_rank.species
Women’s Health lcsh:Medicine Atopobium vaginae Microbiology Prevotella bivia Anaerobic growth General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Gardnerella Lactobacillus iners Gynecology and Obstetrics 030304 developmental biology 0303 health sciences Science & Technology biology 030306 microbiology Chemistry ved/biology General Neuroscience Peptostreptococcus anaerobius lcsh:R Biofilm General Medicine Bacterial vaginosis Ascorbic acid biology.organism_classification BV-associated anaerobes 3. Good health Mobiluncus curtisii Biofilms General Agricultural and Biological Sciences |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP PeerJ, Vol 8, p e9917 (2020) PeerJ |
| Popis: | Background Bacterial vaginosis (BV) is one of the most common vaginal infections worldwide. It is associated with the presence of a dense polymicrobial biofilm on the vaginal epithelium, formed mainly by Gardnerella species. The biofilm also contains other anaerobic species, but little is known about their role in BV development. Aim To evaluate the influence of different culture media on the planktonic and biofilm growth of six cultivable anaerobes frequently associated with BV, namely Gardnerella sp., Atopobium vaginae, Lactobacillus iners, Mobiluncus curtisii, Peptostreptococcus anaerobius and Prevotella bivia. Methods A total of nine different culture media compositions, including commercially available and chemically defined media simulating genital tract secretions, were tested in this study. Planktonic cultures and biofilms were grown under anaerobic conditions (10% carbon dioxide, 10% helium and 80% nitrogen). Planktonic growth was assessed by optical density measurements, and biofilm formation was quantified by crystal violet staining. Results Significant planktonic growth was observed for Gardnerella sp., A. vaginae and L. iners in New York City III broth, with or without ascorbic acid supplementation. Biofilm quantification showed high in vitro biofilm growth for Gardnerella sp., P. anaerobius and P. bivia in almost all culture media excluding Brucella broth. Contrary, only New York City III broth was able to promote biofilm formation for A. vaginae, L. iners and M. curtisii. Conclusions Our data demonstrate that New York City III broth relative to the other tested media is the most conducive for future studies addressing polymicrobial biofilms development as this culture medium allowed the formation of significant levels of single-species biofilms. This work was supported by the research project [PTDC/BIA-MIC/28271/2017] under the scope of COMPETE 2020 [POCI-01-0145-FEDER-028271], supported by the Portuguese Foundation for Science and Technology (FCT), and by the strategic funding of unit [UIDB/04469/2020]. Aliona S. Rosca received financial support from individual Grant [PD/BD/128037/2016]. Nuno Cerca received support from the National Institute of Allergy and Infectious Diseases (R01AI146065-01A1, granted to Christina A. Muzny, MD, MSPH). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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