Antibacterial and Mode of Action of Extracts from Endophytic Fungi Derived from Terminalia mantaly, Terminalia catappa, and Cananga odorata

Autor:	Fred Djague, Rufin Marie Kouipou Toghueo, Norbert Sewald, Fabrice Fekam Boyom, Victorine Lorette Yimgang, Michele Ines Kanko Mbekou, Bruno Njakou Lenta, Darline Dize
Rok vydání:	2021
Předmět:	0106 biological sciences Cell Membrane Permeability food.ingredient Article Subject DPPH Microbial Sensitivity Tests Biology 01 natural sciences Antioxidants General Biochemistry Genetics and Molecular Biology Plant use of endophytic fungi in defense 03 medical and health sciences chemistry.chemical_compound food Picrates Endophytes Cananga Mode of action Medicinal plants 030304 developmental biology 0303 health sciences Cananga odorata Bacteria Cell Death General Immunology and Microbiology Traditional medicine Nucleotides Biphenyl Compounds Cell Membrane Broth microdilution Fungi Terminalia Salt Tolerance General Medicine Catalase biology.organism_classification Anti-Bacterial Agents chemistry Medicine Research Article 010606 plant biology & botany
Zdroj:	BioMed Research International, Vol 2021 (2021) BioMed Research International
ISSN:	2314-6141 2314-6133
DOI:	10.1155/2021/6697973
Popis:	Emerging drug-resistant bacteria creates an urgent need to search for antibiotics drugs with novel mechanisms of action. Endophytes have established a reputation as a source of structurally novel secondary metabolites with a wide range of biological activities. In the present study, we explore the antibacterial potential of endophytic fungi isolated from different tissues of Terminalia mantaly, Terminalia catappa, and Cananga odorata. The crude ethyl acetate extracts of 56 different endophytic fungi were screened against seven bacterial strains using the broth microdilution method. The antibacterial modes of action of the most active extracts (04) were evaluated using E. coli ATCC 25922 and H. influenzae ATCC 49247 strains. Both the DPPH and FRAP assays were used to investigate their antioxidant activity, and their cytotoxicity against the Vero cell line was evaluated using the MTT assay. Out of the 56 crude extracts tested, about 13% were considered very active, 66% partially active, and 21% nonactive against all tested bacterial strains with MIC values ranging from 0.32 μg/mL to 25 μg/mL. The four more potent extracts (MIC I C 50 ranging from 150.71 to 936.08 μg/mL. Three of the four potent extracts were noncytotoxic against the Vero cells line ( C C 50 > 100 μg/mL). Results from this investigation demonstrated that endophytes from Cameroonian medicinal plants might content potent antibacterial metabolites. The bioguided fractionation of these potent extracts is ongoing to isolate and characterise potential active ingredients.
Databáze:	OpenAIRE
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