Arctium lappa Root Extract Prevents Lead-Induced Liver Injury by Attenuating Oxidative Stress and Inflammation, and Activating Akt/GSK-3β Signaling
| Autor: | Laila M. Fadda, Enas A. Zakaria, Naglaa F. El Orabi, Abeer M. Alenazi, Ayman M. Mahmoud, Ahlam M Alhusaini, Hanaa M. Ali, Iman H. Hasan, Amira M. Badr |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Physiology Clinical Biochemistry Pharmacology medicine.disease_cause Biochemistry Article Nitric oxide 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine GSK-3 medicine burdock oxidative stress Molecular Biology Protein kinase B Liver injury lead GSK-3β Cell Biology medicine.disease 030104 developmental biology chemistry Lead acetate 030220 oncology & carcinogenesis Arctium lappa DNA damage Liver function Oxidative stress |
| Zdroj: | Antioxidants Volume 8 Issue 12 |
| ISSN: | 2076-3921 |
| Popis: | Arctium lappa L (A. lappa) is a popular medicinal plant with promising hepatoprotective activity. This study investigated the protective effect of A. lappa root extract (ALRE) on lead (Pb) hepatotoxicity, pointing to its ability to modulate oxidative stress, inflammation, and protein kinase B/Akt/glycogen synthase kinase (GSK)-3&beta signaling. Rats received 50 mg/kg lead acetate (Pb(Ac)2) and 200 mg/kg ALRE or vitamin C (Vit. C) for 7 days, and blood and liver samples were collected. Pb(Ac)2 provoked hepatotoxicity manifested by elevated serum transaminases and lactate dehydrogenase, and decreased total protein. Histopathological alterations, including distorted lobular hepatic architecture, microsteatotic changes, congestion, and massive necrosis were observed in Pb(II)-induced rats. ALRE ameliorated liver function and prevented all histological alterations. Pb(II) increased hepatic lipid peroxidation (LPO), nitric oxide (NO), caspase-3, and DNA fragmentation, and serum C-reactive protein, tumor necrosis factor-&alpha and interleukin-1&beta Cellular antioxidants, and Akt and GSK-3&beta phosphorylation levels were decreased in the liver of Pb(II)-induced rats. ALRE ameliorated LPO, NO, caspase-3, DNA fragmentation and inflammatory mediators, and boosted antioxidant defenses in Pb(II)-induced rats. In addition, ALRE activated Akt and inhibited GSK-3&beta in the liver of Pb(II)-induced rats. In conclusion, ALRE inhibits liver injury in Pb(II)-intoxicated rats by attenuating oxidative injury and inflammation, and activation of Akt/GSK-3&beta signaling pathway. |
| Databáze: | OpenAIRE |
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