Distribution of plasmid-mediated quinolone resistance in Gram-negative bacteria from a tertiary hospital in Nigeria
| Autor: | Armstrong Oyebode Alli, DO Ogbolu, Afolabi Oluwadun, Oluwole Adebayo Daini, Michael C Anorue |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Microbiology (medical)
Gram-negative bacteria Gene Transfer Horizontal plasmid-mediated quinolone resistance medicine.drug_class lcsh:QR1-502 Nigeria Microbial Sensitivity Tests Quinolones lcsh:Microbiology Pathology and Forensic Medicine Microbiology resistance Tertiary Care Centers Plasmid quinolone Genotype Drug Resistance Bacterial Gram-Negative Bacteria lcsh:Pathology medicine Prevalence Humans Typing Replicon Genetics biology Genetic Variation General Medicine Quinolone biology.organism_classification RAPD Anti-Bacterial Agents Random Amplified Polymorphic DNA Technique Molecular Typing Genes Bacterial Conjugation Genetic Gram-Negative Bacterial Infections Bacteria lcsh:RB1-214 Plasmids |
| Zdroj: | Indian Journal of Pathology and Microbiology, Vol 59, Iss 3, Pp 322-326 (2016) |
| ISSN: | 0974-5130 |
| Popis: | Background: Until recently, mechanisms of resistance to quinolones in Gram-negative bacteria were believed to be only chromosome encoded. However, emergence of plasmid-mediated quinolone resistance (PMQR) has been reported worldwide. Aim: This study investigated distribution of PMQR in Gram-negative bacteria from a tertiary hospital in eastern part of Nigeria. Materials and Methods: Seventy-one nonduplicate Gram-negative bacterial isolates of eight species were analyzed for antimicrobial susceptibility, genotypic detection of various PMQRs, typed by random amplified polymorphic DNA (RAPD) and analysis of plasmids present, including replicon typing. Results: The minimum inhibitory concentrations showed MIC 90 values as high as 256 μg/ml for fluoroquinolones. Carriage of PMQR was found to be 35.2%. Twenty (28.2%) isolates carried various qnr genes, of which seven (9.9%) qnrA1 ; four (5.6%) qnrB1 ; eight (11.3%) qnrS1 while one (1.4%) encoded qnrD1 . Eighteen (25.4%) isolates were positive for aac(6')-Ib-cr while carriage of multiple genes exists in some strains. Similarly, 13 isolates (18.7%) were found to carry PMQR efflux pump gene, qepA . Conjugation experiments revealed that the plasmids once transferred coded for fluoroquinolone resistance. The transconjugant strains carried a common plasmid estimated to be 65 kb. These plasmids were untypable for replicon/incompatibility. Typing revealed high diversity among all species tested with no identical RAPD pattern seen. Conclusion: This study further confirms high level resistance to many antimicrobials in different species of Gram-negative bacteria including fluoroquinolones and spread of PMQR genes in Southern Nigeria. |
| Databáze: | OpenAIRE |
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