Tumor-Infiltrating T Cells Concurrently Overexpress CD200R with Immune Checkpoints PD-1, CTLA-4, and TIM-3 in Non-Small-Cell Lung Cancer
| Autor: | Masahiro Tsuboi, Yinghan Su, Jun Suzuki, Toshiaki Yoshikawa, Shota Yamazaki, Ryo Morisue, Atsushi Ochiai, Tetsuya Nakatsura, Genichiro Ishii |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Lung Neoplasms
T cell Programmed Cell Death 1 Receptor Pathology and Forensic Medicine 03 medical and health sciences Lymphocytes Tumor-Infiltrating 0302 clinical medicine Immune system Orexin Receptors Carcinoma Non-Small-Cell Lung Biomarkers Tumor medicine Humans Cytotoxic T cell CTLA-4 Antigen Lung cancer Receptor Hepatitis A Virus Cellular Receptor 2 Molecular Biology biology Chemistry Cell Biology General Medicine medicine.disease Immune checkpoint Up-Regulation medicine.anatomical_structure CTLA-4 030220 oncology & carcinogenesis biology.protein Cancer research Antibody 030215 immunology |
| Zdroj: | Pathobiology. 88:218-227 |
| ISSN: | 1423-0291 1015-2008 |
| DOI: | 10.1159/000511557 |
| Popis: | Introduction: CD200R has been reported to be the receptor for the immune checkpoint molecule CD200 and can transduce immune-suppressive signals. In this study, we mainly focused on the expression level of CD200R in T cells in pulmonary artery (PA) blood and non-small-cell lung cancer (NSCLC) tumor tissue. Methods: Immune cells were isolated from dissected tumor samples and PA blood of NSCLC patients and analyzed with multiparameter flow cytometry. The co-expression of CD200R with other immune checkpoints, including programmed cell death protein 1 (PD-1), cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), and T cell immunoglobulin and mucin domain-containing protein 3 (TIM-3), was also investigated. Results: CD200R expression was observed on the surface of approximately 75% of T cells among tumor-infiltrating leukocytes (TILs). Compared to T cells extracted from TILs, only 55% of T cells extracted from PA blood exhibited CD200R expression. Moreover, with higher expression of CD200R, the expression of other immune checkpoints, including PD-1, CTLA-4, and TIM-3, was also increased in tumor-infiltrating T cells compared to T cells in PA blood. Conclusions: Our results showed that those tumors were dominated by T cells expressing CD200R together with other checkpoints, which suggests a phenotypic change after T cell infiltration into the tumor, such as T cell exhaustion. |
| Databáze: | OpenAIRE |
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