A Genetic Interaction Map of RNA-Processing Factors Reveals Links between Sem1/Dss1-Containing Complexes and mRNA Export and Splicing
| Autor: | Hannes Braberg, Gerard Cagney, Tracy L. Kress, Trey Ideker, Gregg B. Whitworth, Sean R. Collins, Jonathan S. Weissman, Christine Guthrie, Gwendolyn M. Wilmes, Sourav Bandyopadhyay, Michael Shales, Nevan J. Krogan, Megan Bergkessel |
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| Rok vydání: | 2008 |
| Předmět: |
Proteasome Endopeptidase Complex
Saccharomyces cerevisiae Proteins RNA Splicing Saccharomyces cerevisiae Computational biology Models Biological RNA Transport Article Protein Interaction Mapping RNA Messenger RNA Processing Post-Transcriptional COP9 signalosome Molecular Biology Gene Genetics Messenger RNA biology COP9 Signalosome Complex RNA Cell Biology biology.organism_classification Multiprotein Complexes Exoribonucleases RNA splicing Epistasis Peptide Hydrolases |
| Zdroj: | Molecular Cell. 32:735-746 |
| ISSN: | 1097-2765 |
| Popis: | We used a quantitative, high-density genetic interaction map, or E-MAP (Epistatic MiniArray Profile), to interrogate the relationships within and between RNA processing pathways. Due to their complexity, and the essential roles of many of the components, these pathways have been difficult to functionally dissect. Here we report the results for 107,155 individual interactions involving 552 mutations, 166 of which are hypomorphic alleles of essential genes. Our data enabled the discovery of links between components of the mRNA export and splicing machineries and Sem1/Dss1, a component of the 19S proteasome. In particular, we demonstrate that Sem1 has a proteasome-independent role in mRNA export as a functional component of the Sac3-Thp1 complex. Sem1 also interacts with Csn12, a component of the COP9 signalosome. Finally, we show that Csn12 plays a role in pre-mRNA splicing, which is independent of other signalosome components. Thus, Sem1 is involved in three separate and functionally distinct complexes. |
| Databáze: | OpenAIRE |
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