The effect of inhibiting exosomes derived from adipose-derived stem cells via the TGF-β1/Smad pathway on the fibrosis of keloid fibroblasts
| Autor: | Hui-Jun Zhang, Yucang Shi, Zhan-Peng Li, Zhi-Yuan Wu, Si-Mu Liao, Zhi-Hong Zhou, Zeyong Wu, Haihua Huang |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
030102 biochemistry & molecular biology medicine.diagnostic_test business.industry Cell Adipose tissue Cell migration medicine.disease Molecular biology Flow cytometry 03 medical and health sciences 030104 developmental biology Keloid medicine.anatomical_structure Apoptosis Fibrosis Medicine Original Article Surgery Stem cell business |
| Zdroj: | Gland Surg |
| ISSN: | 2227-8575 2227-684X |
| DOI: | 10.21037/gs-21-4 |
| Popis: | BACKGROUND: The main mechanism of keloid formation is that keloid fibroblasts (KFs) apoptosis is inhibited, leading to excessive proliferation. Transforming growth factor-β1 (TGF-β1) is a key signal molecule in the process of regulating cell fibrosis. This paper discusses the effect of adipose-derived stem cell exosomes (ADSCs-EXO) on the proliferation and apoptosis of KFS and its possible mechanism, in order to provide reference for the clinical intervention of hypertrophic scar. METHODS: ADSCs were isolated and cultured from human adipose tissue, the supernatant was collected, and the exosomes secreted by ADSCs-EXO were extracted by ultracentrifugation. At the same time, KFs were cultured from human keloid tissue to P3 generation, and then divided into four groups: control group, experimental group A, experimental group B and experimental group C. KFs were then cultured with four concentrations of ADSCs-EXO (0, 1, 10, and 100 µg/mL, respectively). After 24 hours, cells in each group were taken to detect the following: proliferation of cells in each group using the cell counting Kit 8 (CCK-8) method, cell migration ability via the Transwell test, cell apoptosis by flow cytometry, collagen synthesis using the hydroxyproline method, messenger ribonucleic acid (mRNA) expression of fibrosis-related genes in each group by real-time fluorescent polymerase chain amplification, and the expression of fibrosis-related proteins in the cells of each group by western blotting. RESULTS: Compared with the control group, the proliferation rate, migration rate, and collagen synthesis levels in the three experimental groups decreased with the increase of ADSCs-EXO concentration, while the apoptosis rate in the three experimental groups increased with the increase of ADSCs-EXO concentration, and the differences were statistically significant (P |
| Databáze: | OpenAIRE |
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