New Cytoplasmic Virus-Like Elements (VLEs) in the Yeast Debaryomyces hansenii
| Autor: | Cécile Neuvéglise, Serge Casaregola, Barbara Żarowska, Xymena Połomska, Joanna Zyzak, Zbigniew Lazar |
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| Přispěvatelé: | Department of Biotechnology and Food Microbiology, Wroclaw University of Environmental and Life Sciences, Sciences Pour l'Oenologie (SPO), Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Ludwik Hirszfeld Institute of Immunology and Experimental therapy (LUDWIK HIRSZFELD INSTITUTE OF IMMUNOLOGY AND EXPERIMENTAL THERAPY), Polska Akademia Nauk = Polish Academy of Sciences (PAN), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Debaryomyces hansenii
Health Toxicology and Mutagenesis killer activity [SDV]Life Sciences [q-bio] yeast Toxicology virus-like elements (VLEs) 03 medical and health sciences killer toxins ORFS Killer yeast Gene 030304 developmental biology Genetics 0303 health sciences biology 030306 microbiology Yarrowia Penicillium roqueforti Deoxyribonuclease biology.organism_classification Yeast linear dsDNA plasmids Medicine osmotolerance |
| Zdroj: | Toxins Toxins, MDPI, 2021, 13 (9), pp.615. ⟨10.3390/toxins13090615⟩ Volume 13 Issue 9 Toxins, Vol 13, Iss 615, p 615 (2021) |
| ISSN: | 2072-6651 |
| Popis: | Yeasts can have additional genetic information in the form of cytoplasmic linear dsDNA molecules called virus-like elements (VLEs). Some of them encode killer toxins. The aim of this work was to investigate the prevalence of such elements in D. hansenii killer yeast deposited in culture collections as well as in strains freshly isolated from blue cheeses. Possible benefits to the host from harboring such VLEs were analyzed. VLEs occurred frequently among fresh D. hansenii isolates (15/60 strains), as opposed to strains obtained from culture collections (0/75 strains). Eight new different systems were identified: four composed of two elements and four of three elements. Full sequences of three new VLE systems obtained by NGS revealed extremely high conservation among the largest molecules in these systems except for one ORF, probably encoding a protein resembling immunity determinant to killer toxins of VLE origin in other yeast species. ORFs that could be potentially involved in killer activity due to similarity to genes encoding proteins with domains of chitin-binding/digesting and deoxyribonuclease NucA/NucB activity, could be distinguished in smaller molecules. However, the discovered VLEs were not involved in the biocontrol of Yarrowia lipolytica and Penicillium roqueforti present in blue cheeses. |
| Databáze: | OpenAIRE |
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