Induction of multidrug resistance (MDR) by transfection of MCF-10A cell line with c-Ha-ras and c-erbB-2 oncogenes
| Autor: | Antonietta Raffaella Maria Sabbatini, Simonetta Calvo, Lisa Fiore, Letizia Mattii, Paola Valentini, Mario Petrini, Fortunato Ciardiello, Fulvio Basolo |
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| Přispěvatelé: | Sabbatini, Ar, Basolo, F, Valentini, P, Mattii, L, Calvo, S, Fiore, L, Ciardiello, Fortunato, Petrini, M. |
| Jazyk: | angličtina |
| Rok vydání: | 1994 |
| Předmět: |
Cancer Research
C erbb 2 animal structures Molecular Sequence Data Gene Expression Breast Neoplasms Transfection Cell membrane C ha ras Tumor Cells Cultured medicine Humans ATP Binding Cassette Transporter Subfamily B Member 1 skin and connective tissue diseases Base Sequence Chemistry Genes erbB-2 Immunohistochemistry Molecular biology Drug Resistance Multiple Epithelium Multiple drug resistance Genes ras Phenotype medicine.anatomical_structure Verapamil Oncology Doxorubicin Cell culture Drug Screening Assays Antitumor medicine.drug |
| Popis: | To investigate the relationship between oncogene activation and appearance of multidrug resistance (MDR) we transfected the human breast epithelial cell line MCF-10A, negative for the expression of the P-glycoprotein, with c-Ha-ras and/or c-erbB-2 oncogenes. The appearance of the MDR phenotype was then studied by evaluating mdr-1 mRNA expression, the presence of P-glycoprotein on the cell membrane and the onset of doxorubicin resistance, together with the effect of the reversing agent verapamil. We found that only MCF-10A transfected with both c-Ha-ras and c-erbB-2 oncogenes acquired the MDR phenotype. |
| Databáze: | OpenAIRE |
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