Fruit-specific RNAi-mediated suppression of DET1 enhances carotenoid and flavonoid content in tomatoes
| Autor: | Henk M. J. Pennings, Peter M. Bramley, Stephen R. King, David A. Brummell, Diane Burgess, Alessandro Manfredonia, Paul D. Fraser, Chris Bowler, John Uhlig, Ageeth van Tuinen, Ganga Rao Davuluri, Robert Newman, Joe Palys |
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| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Transgene
Flavonoid Biomedical Engineering Down-Regulation Bioengineering Biology Applied Microbiology and Biotechnology Article Solanum lycopersicum RNA interference Gene Expression Regulation Plant Botany Gene silencing Gene Carotenoid Regulator gene Plant Proteins chemistry.chemical_classification Regulation of gene expression Flavonoids fungi food and beverages Plants Genetically Modified Carotenoids chemistry Biochemistry Molecular Medicine RNA Interference Nutritive Value Biotechnology |
| Popis: | Tomatoes are a principal dietary source of carotenoids and flavonoids, both of which are highly beneficial for human health1,2. Overexpression of genes encoding biosynthetic enzymes or transcription factors have resulted in tomatoes with improved carotenoid or flavonoid content, but never with both3–7. We attempted to increase tomato fruit nutritional value by suppressing an endogenous photomorphogenesis regulatory gene, DET1, using fruit-specific promoters combined with RNA interference (RNAi) technology. Molecular analysis indicated that DET1 transcripts were indeed specifically degraded in transgenic fruits. Both carotenoid and flavonoid contents were increased significantly, whereas other parameters of fruit quality were largely unchanged. These results demonstrate that manipulation of a plant regulatory gene can simultaneously influence the production of several phytonutrients generated from independent biosynthetic pathways, and provide a novel example of the use of organ-specific gene silencing to improve the nutritional value of plant-derived products. |
| Databáze: | OpenAIRE |
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