Functional role of respiratory supercomplexes in mice: SCAF1 relevance and segmentation of the Qpool
| Autor: | José Antonio Enríquez, Jesús R. Huertas, Jesús Vázquez, Enrique Calvo, Sara Cogliati, Adela Guarás, Rafael A. Casuso, Rebeca Acín-Pérez, Pablo Hernansanz-Agustín, Yolanda Martí-Mateos, Marta Loureiro-López, Fernando García-Marqués, Juan Carlos Silla-Castro, Marta Carro-Alvarellos |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Ministerio de Ciencia e Innovación (España), Centro de Investigación Biomedica en Red - CIBER, Instituto de Salud Carlos III, Fundación La Marató TV3, Fundación La Caixa, Fundación ProCNIC, Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF), Instituto de Salud Carlos III - ISCIII, Fundació La Marató, European Regional Development Fund (ERDF/FEDER) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Functional role
chemistry.chemical_classification 0303 health sciences Reactive oxygen species Multidisciplinary Functional analysis Chemistry SciAdv r-articles Proteomics Biochemistry Cell biology 03 medical and health sciences 0302 clinical medicine Exercise performance Respirasome Respiration Respiratory system Molecular Biology Research Articles 030217 neurology & neurosurgery Research Article 030304 developmental biology |
| Zdroj: | Repisalud Instituto de Salud Carlos III (ISCIII) Digibug. Repositorio Institucional de la Universidad de Granada instname Digibug: Repositorio Institucional de la Universidad de Granada Universidad de Granada (UGR) Science Advances |
| ISSN: | 2018-0993 |
| Popis: | Mitochondrial respiratory complexes assemble into supercomplexes (SC). Q-respirasome (III2 + IV) requires the supercomplex assembly factor (SCAF1) protein. The role of this factor in the N-respirasome (I + III2 + IV) and the physiological role of SCs are controversial. Here, we study C57BL/6J mice harboring nonfunctional SCAF1, the full knockout for SCAF1, or the wild-type version of the protein and found that exercise performance is SCAF1 dependent. By combining quantitative data–independent proteomics, 2D Blue native gel electrophoresis, and functional analysis of enriched respirasome fractions, we show that SCAF1 confers structural attachment between III2 and IV within the N-respirasome, increases NADH-dependent respiration, and reduces reactive oxygen species (ROS). Furthermore, the expression of AOX in cells and mice confirms that CI-CIII superassembly segments the CoQ in two pools and modulates CI-NADH oxidative capacity MINECO SAF2015-65633-R MCIU RTI2018-099357-B-I00 CIBERFES CB16/10/00282 Human Frontier Science Program RGP0016/2018 ISCIII-SGEFI/FEDER, ProteoRed ISCIII-IPT13/0001 Fundacio MaratoTV3 122/C/2015 La Caixa Foundation HR17-00247 Ministry of Economy, Industry and Competitiveness (MEIC) Pro-CNIC Foundation MINECO award SEV-2015-0505 MINECO-BIO2015-67580-P PGC2018-097019-B-I00 |
| Databáze: | OpenAIRE |
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