An immunosensor based on magnetic relaxation switch and polystyrene microparticle-induced immune multivalency enrichment system for the detection of Pantoea stewartii subsp. Stewartii
| Autor: | Cai Qi, Ming qiang Zou, Da ning Wang, Yi-Ping Chen, Qiang Xue, Yong liang Li, Meng xia Xie |
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| Rok vydání: | 2012 |
| Předmět: |
Relative standard deviation
Biomedical Engineering Biophysics Analytical chemistry Biosensing Techniques chemistry.chemical_compound Immune system Electrochemistry Magnetic relaxation Pantoea stewartii subsp stewartii Microparticle Detection limit Immunoassay Chromatography biology Chemistry Immunomagnetic Separation Pantoea General Medicine Equipment Design biology.organism_classification Bacterial Load Microspheres Equipment Failure Analysis Polystyrenes Polystyrene Biotechnology |
| Zdroj: | Biosensorsbioelectronics. 43 |
| ISSN: | 1873-4235 |
| Popis: | A rapid, sensitive, and simple immunosensor has been developed for the detection of Pantoea stewartii subsp. Stewartii (Pss). This immunosensor combines magnetic relaxation switch (MRS) assay with polystyrene microparticle-induced immune multivalency enrichment system. Comparing to conventional enzyme-linked immunosorbent assay (ELISA), the immunosensor developed in this study provides higher sensitivity and requires less analysis time. The detection limit of Pss obtained by immunosensor was determined to be 103 cfu/mL, 50 times lower than that by ELISA (5×104 cfu/mL), while the analysis time required by immunosensor is 30 min much shorter than that by ELISA. The average recoveries studied with Pss at various spiking levels ranged from 85.5% to 93.4% with a relative standard deviation (RSD) below 6.0%. No cross-reaction with the other five strains was found, demonstrating a good specificity of Pss detection. The results showed that the MRS immunosensor combined with PS-induced immune multivalency enhancement system is a promising platform for the determination of large biological molecules due to its high sensitivity, specificity, homogeneity, and speed. |
| Databáze: | OpenAIRE |
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