Induction of RNA-mediated resistance to papaya ringspot virus type W
| Autor: | Sunee Kertbundit, Mila Juricek, Pongrit Krubphachaya |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Small RNA
Potyvirus Biochemistry Polymerase Chain Reaction Papaya ringspot virus Caulimovirus Cucumis melo Gene silencing Coding region RNA Small Interfering Promoter Regions Genetic Molecular Biology Gene Southern blot Plant Diseases biology Models Genetic RNA General Medicine biology.organism_classification Blotting Northern Plants Genetically Modified Virology Molecular biology Immunity Innate Transformation (genetics) Blotting Southern Capsid Proteins |
| Zdroj: | Journal of biochemistry and molecular biology. 40(3) |
| ISSN: | 1225-8687 |
| Popis: | Transformation of cantaloupes with the coat protein (cp) gene of papaya ringspot virus type W (PRSV-W), Thai isolate, was used to introduce virus resistance. Binary vectors containing either the full length coat protein coding region under control of the 35S CaMV promoter(pSA1175), or the inverted-repeat of a coat protein coding region (pSA1304), were constructed and used for Agrobacterium-mediated transformation of cotyledonary explants of the cantaloupe cultivar Sun Lady. Four independent transgenic lines were obtained using pSA1304 and one using pSA1175. Integration of the PRSV-W cp gene into the genome of these transgenic lines was verified by PCR amplification, GUS assays and Southern blot hybridization. In vitro inoculation of these lines with PRSV-W revealed that whereas the line containing pSA1175 remained sensitive, the four lines containing pSA1304 were resistant. The presence of small RNA species, presumably siRNA, corresponding to regions of the viral cp gene in transgenic lines resistant to PRSV-W supports the involvement of post-transcriptional gene silencing in the establishment of resistance. |
| Databáze: | OpenAIRE |
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