Mitogen-Activated Protein Kinase 4-Regulated Metabolic Networks
Autor: | Chuwei Lin, Aneirin Alan Lott, Wei Zhu, Craig P. Dufresne, Sixue Chen |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2022 |
Předmět: |
Arabidopsis thaliana
Proteome QH301-705.5 Arabidopsis cytokinesis Article Gene Expression Regulation Enzymologic Catalysis Inorganic Chemistry Gene Expression Regulation Plant Metabolomics Physical and Theoretical Chemistry Biology (General) Molecular Biology QD1-999 Spectroscopy mitogen-activated protein kinase 4 metabolomics and proteomics immunity polyamine metabolic networks Gene Expression Profiling Organic Chemistry General Medicine Computer Science Applications Chemistry Phenotype Mutation Metabolome Energy Metabolism Transcriptome Metabolic Networks and Pathways RNA Helicases |
Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 2; Pages: 880 International Journal of Molecular Sciences, Vol 23, Iss 880, p 880 (2022) International Journal of Molecular Sciences |
ISSN: | 1422-0067 |
DOI: | 10.3390/ijms23020880 |
Popis: | Mitogen-activated protein kinase 4 (MPK4) was first identified as a negative regulator of systemic acquired resistance. It is also an important kinase involved in many other biological processes in plants, including cytokinesis, reproduction, and photosynthesis. Arabidopsis thaliana mpk4 mutant is dwarf and sterile. Previous omics studies including genomics, transcriptomics, and proteomics have revealed new functions of MPK4 in different biological processes. However, due to challenges in metabolomics, no study has touched upon the metabolomic profiles of the mpk4 mutant. What metabolites and metabolic pathways are potentially regulated by MPK4 are not known. Metabolites are crucial components of plants, and they play important roles in plant growth and development, signaling, and defense. Here we used targeted and untargeted metabolomics to profile metabolites in the wild type and the mpk4 mutant. We found that in addition to the jasmonic acid and salicylic acid pathways, MPK4 is involved in polyamine synthesis and photosynthesis. In addition, we also conducted label-free proteomics of the two genotypes. The integration of metabolomics and proteomics data allows for an insight into the metabolomic networks that are potentially regulated by MPK4. |
Databáze: | OpenAIRE |
Externí odkaz: | |
Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |