Development of a Novel Nanoarchitecture of the Robust Photosystem I from a Volcanic Microalga Cyanidioschyzon merolae on Single Layer Graphene for Improved Photocurrent Generation
| Autor: | Margot Jacquet, Tomasz Goral, Ersan Harputlu, Shin-ya Miyagishima, Joanna Kargul, Kasim Ocakoglu, Piotr Wróbel, Miriam Izzo, C. Gokhan Unlu, Radosław Mazur, Takayuki Fujiwara |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
System
Electron-Transfer 02 engineering and technology 01 natural sciences law.invention law Biology (General) Spectroscopy single layer graphene biohybrid nanodevices biology General Medicine 021001 nanoscience & nanotechnology Computer Science Applications Chemistry Cyanidioschyzon merolae Mechanism 0210 nano-technology Cobalt Materials science photosystem I QH301-705.5 chemistry.chemical_element Nanotechnology 010402 general chemistry Photosystem I Redox Catalysis Inorganic Chemistry Red Alga Electron transfer Immobilization Fabrication Orientation Monolayer direct electron transfer Physical and Theoretical Chemistry QD1-999 Molecular Biology Photocurrent Graphene Organic Chemistry biophotovoltaics Proteins biology.organism_classification Recombination 0104 chemical sciences chemistry |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 16 International Journal of Molecular Sciences, Vol 22, Iss 8396, p 8396 (2021) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms22168396 |
| Popis: | Here, we report the development of a novel photoactive biomolecular nanoarchitecture based on the genetically engineered extremophilic photosystem I (PSI) biophotocatalyst interfaced with a single layer graphene via pyrene-nitrilotriacetic acid self-assembled monolayer (SAM). For the oriented and stable immobilization of the PSI biophotocatalyst, an His6-tag was genetically engineered at the N-terminus of the stromal PsaD subunit of PSI, allowing for the preferential binding of this photoactive complex with its reducing side towards the graphene monolayer. This approach yielded a novel robust and ordered nanoarchitecture designed to generate an efficient direct electron transfer pathway between graphene, the metal redox center in the organic SAM and the photo-oxidized PSI biocatalyst. The nanosystem yielded an overall current output of 16.5 µA·cm−2 for the nickel- and 17.3 µA·cm−2 for the cobalt-based nanoassemblies, and was stable for at least 1 h of continuous standard illumination. The novel green nanosystem described in this work carries the high potential for future applications due to its robustness, highly ordered and simple architecture characterized by the high biophotocatalyst loading as well as simplicity of manufacturing. |
| Databáze: | OpenAIRE |
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