Identification of mycobacteria and other acid fast organisms associated with pulmonary disease
| Autor: | Bassirou Diarra, C Lekuk, Agatha Ani, Anou M. Somboro, John Idoko, Tounkara Anatole, Ulf R Dahle, F Yetunde |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Microbiology (medical)
Mycobacterium kansasii Mycobacterium bovis Other mycobacteria species lcsh:Arctic medicine. Tropical medicine Acid fast bacilli lcsh:RC955-962 lcsh:R Mycobacteria HIV lcsh:Medicine Mycobacterium tuberculosis Biology biology.organism_classification Mycobacterium caprae bacterial infections and mycoses Microbiology Infectious Diseases Mycobacterium microti Acid fast organism Pulmonary disease Mycobacterium africanum Mycobacterium Mycobacterium canetti |
| Zdroj: | Asian Pacific Journal of Tropical Disease, Vol 1, Iss 4, Pp 259-262 (2011) |
| ISSN: | 2222-1808 |
| Popis: | Objective To identify Mycobacterium tuberculosis (M. tuberculosis) and other acid fast organisms isolated from sputum of HIV positive adult patients with pulmonary disease in Jos, Nigeria. Methods Acid fast organisms isolated from 80 acid fast bacilli (AFB) positive sputa of HIV positive adult patients suspected for tuberculosis in Jos, Nigeria were identified for members of M. tuberculosis complex (M. tuberculosis, Mycobacterium bovis, Mycobacterium africanum, Mycobacterium canetti, Mycobacterium microti and Mycobacterium caprae) by use of spoligootyping, Multiplex Gen Probe, Hain genotype assay and gene sequencing for spoligotype negative isolates. Results Seven different spoligotypes of M. tuberculosis complex were identified from 70/80 (87.5%) total number of isolates. Mycobacterium kansasii (1), Mycobacterium dulvalii (1) Nocardia species (1) and Tsukamurella species (2) were detected from 5/10 spoligotype negative isolates. Conclusions Although M. tuberculosis is the dominant AFB associated with chronic pulmonary disease in Jos, Nigeria, other clinically relevant mycobacteria were also observed in the study. This suggests that other AFB positive microorganisms associated with tuberculosis-like symptoms might be misdiagnosed and incorrectly treated as M. tuberculosis. It is therefore necessary for laboratories in tuberculosis high burden countries to step up diagnostic procedures beyond routine smear microscopy. |
| Databáze: | OpenAIRE |
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