Guanylyl cyclase C is a selective marker for metastatic colorectal tumors in human extraintestinal tissues
| Autor: | Michael T. Barber, Scott A. Waldman, Scott D. Goldstein, Sanjoy Biswas, P. K. Park, S. L. Carrithers, Scott J. Parkinson |
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| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
Receptors Peptide Colorectal cancer Guanylin Receptors Enterotoxin Biology Polymerase Chain Reaction chemistry.chemical_compound Intestinal mucosa Internal medicine Biomarkers Tumor medicine Humans Northern blot Neoplasm Metastasis Receptor Multidisciplinary Guanylate cyclase 2C Biological Sciences medicine.disease Endocrinology Receptors Guanylate Cyclase-Coupled chemistry Guanylate Cyclase Cancer cell Cancer research Colorectal Neoplasms Uroguanylin |
| Zdroj: | Proceedings of the National Academy of Sciences. 93:14827-14832 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.93.25.14827 |
| Popis: | Guanylyl cyclase C (GCC) has been detected only in intestinal mucosa and colon carcinoma cells of placental mammals. However, this receptor has been identified in several tissues in marsupials, and its expression has been suggested in tissues other than intestine in placental mammals. Selective expression of GCC by colorectal tumor cells in extraintestinal tissues would permit this receptor to be employed as a selective marker for metastatic disease. Thus, expression of GCC was examined in human tissues and tumors, correlating receptor function with detection by PCR. GCC was detected by ligand binding and catalytic activation in normal intestine and primary and metastatic colorectal tumors, but not in extraintestinal tissues or tumors. Similarly, PCR yielded GCC-specific amplification products with specimens from normal intestine and primary and metastatic colorectal tumors, but not from extraintestinal tissues or tumors. Northern blot analysis employing GCC-specific probes revealed an ≈4-kb transcript, corresponding to recombinant GCC, in normal intestine and primary and metastatic colorectal tumors, but not in extraintestinal tissues. Thus, GCC is selectively expressed in intestine and colorectal tumors in humans and appears to be a relatively specific marker for metastatic cancer cells in normal tissues. Indeed, PCR of GCC detected tumor cells in blood from some patients with Dukes B colorectal cancer and all patients examined with Dukes C and D colorectal cancer, but not in that from normal subjects or patients with Dukes A colon carcinoma or other nonmalignant intestinal pathologies. |
| Databáze: | OpenAIRE |
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