Immunological monitoring during therapeutic vaccination as a prerequisite for the design of new effective therapies: induction of a vaccine-specific CD4+ T-cell proliferative response in chronic hepatitis B carriers
| Autor: | Mark Page, Reinhart Zachoval, Norbert H. Grüner, C.A. Schirren, Emma Birtles, Stefan Zeuzem, Gerd R. Pape, Helmut M. Diepolder, John R. Bailey, Tilman Gerlach, Jörg Trojan, W. Schraut, Eve Whitehead, Maria-Christina Jung |
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| Rok vydání: | 2002 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male Interleukin 2 Lymphocyte Activation medicine.disease_cause Virus Interferon-gamma Hepatitis B Chronic Double-Blind Method Orthohepadnavirus Antigen Monitoring Immunologic medicine Humans Hepatitis B Vaccines Hepatitis B virus General Veterinary General Immunology and Microbiology biology Vaccination Public Health Environmental and Occupational Health Alanine Transaminase Middle Aged biology.organism_classification Hepatitis B Core Antigens Virology Interleukin-10 HBcAg Infectious Diseases Hepadnaviridae DNA Viral Immunology Interleukin-2 Molecular Medicine Female Interleukin-5 T-Lymphocytes Cytotoxic medicine.drug |
| Zdroj: | Vaccine. 20:3598-3612 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/s0264-410x(02)00309-2 |
| Popis: | We characterized the anti-viral T-cell response in 22 chronically infected patients, who participated in a European multi-center randomized placebo-controlled, double-blind study therapeutic vaccination trial with pre-S1, pre-S2 and S antigenic components of the hepatitis B virus (HBV). It induced a significant HBsAg-specific T-cell proliferation and the production of Th2-cytokines (i.e. IL-5). A specific induction of Th1-lymphokines was not detectable although this has been demonstrated in this study in response to the nucleocapsid protein (HBcAg). Further analysis indicated that this approach does not activate HBV-specific CD8+ T-lymphocytes as detected by ELISPOT-assay. Our results might explain why a specific therapeutic vaccine, although safe and well-tolerated is not always able to break tolerance leading to the clearance of the hepatitis B virus. |
| Databáze: | OpenAIRE |
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