Efficacy of DNA–hsp65 vaccination for tuberculosis varies with method of DNA introduction in vivo
| Autor: | S.M.A. dos Santos, Rubens R. Dos Santos, Juliana Rodrigues, Izaíra T. Brandão, Karla De Melo Lima, Célio Lopes Silva |
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| Rok vydání: | 2003 |
| Předmět: |
DNA
Bacterial Tuberculosis Chaperonins chemical and pharmacologic phenomena Injections Intramuscular DNA vaccination Gene gun Mycobacterium tuberculosis Mice Bacterial Proteins Vaccines DNA medicine Animals Tuberculosis Vaccines Mice Inbred BALB C General Veterinary General Immunology and Microbiology biology Immunogenicity Public Health Environmental and Occupational Health Chaperonin 60 Biolistics medicine.disease biology.organism_classification Antibodies Bacterial Virology Microspheres Vaccination Infectious Diseases Immunization Naked DNA Immunology Cytokines Molecular Medicine Female Gold |
| Zdroj: | Vaccine. 22:49-56 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/s0264-410x(03)00543-7 |
| Popis: | A DNA vaccine codifying the mycobacterial hsp65 can prevent infection with Mycobacterium tuberculosis in a prophylactic setting and also therapeutically reduce the number of bacteria in infected mice. The protective mechanism is thought to be related to Th1-mediated events that result in bacterial killing. To determine the best method of hsp65 introduction for vaccination efficacy against tuberculosis (TB), we evaluated the immunogenicity and protection of DNA-hsp65 administered by gene gun bombardment or intramuscular (i.m.) injection of naked DNA. Immunization by gene gun induced immune response with plasmid doses 100-fold lower than those required for intramuscular immunization. However, in contrast to intramuscular immunization, which was protective in these studies, gene gun immunization did not protect BALB/c mice against challenge infection. |
| Databáze: | OpenAIRE |
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