Surrogate Thyroglobulin Receptors and T Cell Proliferation in Hashimoto's Thyroiditis
| Autor: | Sandra M. McLachlan, D. I. W. Phillips, N. Fukuma, T. Hoy |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Male
medicine.drug_class T-Lymphocytes Endocrinology Diabetes and Metabolism medicine.medical_treatment T cell Palmitic Acid Receptors Cell Surface Palmitic Acids Monoclonal antibody Thyroglobulin Thyroiditis Endocrinology Antigen medicine Humans Antigen-presenting cell Receptor Fluorescent Dyes Receptors Thyroid Hormone biology Chemistry Cell Membrane Thyroiditis Autoimmune Antibodies Monoclonal Flow Cytometry medicine.disease Molecular biology medicine.anatomical_structure Immunology Leukocytes Mononuclear biology.protein Female Antibody Cell Division Fluorescein-5-isothiocyanate |
| Zdroj: | Thyroid. 2:291-297 |
| ISSN: | 1557-9077 1050-7256 |
| Popis: | Immunoglobulin molecules on the surface of a B lymphocyte are the endogenous "receptors" to which specific antigens bind. Studies in mice have shown that a monoclonal antibody, conjugated with palmitate to provide a lipid tail, can be inserted into the cell membrane to provide a "surrogate" antigen receptor. We have investigated whether a palmitate conjugate of a human monoclonal antibody specific for thyroglobulin (TG) could function as a surrogate TG receptor on blood mononuclear cells separated into fractions enriched for T cells or depleted of T cells (non-T cells). Using flow cytometry, we detected surrogate TG receptors on non-T (but not on T) cells from 11 of 11 individuals studied (5 Hashimoto patients and 6 control donors). In contrast, endogenous TG receptors could only be detected on non-T cells from 1 of 3 Hashimoto patients and from 0 of 4 control donors. Because of the efficient binding of TG by surrogate receptors on non-T cells, we assessed the ability of such cells to present TG to T cells. Proliferation in response to TG was observed in T cells from only 1 of 5 Hashimoto patients. This low frequency of response was no different from that previously detected using cultures of T cells and autologous dendritic cells. Therefore, the successful generation of surrogate receptors on non-T cells is not associated with more efficient TG presentation of T cells. Furthermore, the significance of the present study is that the T cells, not the antigen-presenting cells, are likely to be the limiting element in the T cell proliferative response to TG and other thyroid autoantigens. |
| Databáze: | OpenAIRE |
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